Amplification and overexpression of TGIF2, a novel homeobox gene of the TALE superclass, in ovarian cancer cell lines

被引:94
作者
Imoto, I
Pimkhaokham, A
Watanabe, T
Saito-Ohara, F
Soeda, E
Inazawa, J
机构
[1] Tokyo Med & Dent Univ, Med Res Inst, Dept Mol Cytogenet, Bunkyo Ku, Tokyo 1138510, Japan
[2] Inst Phys & Chem Res, Tsukuba Life Sci Ctr, RIKEN, Gene Bank, Tsukuba, Ibaraki 3050074, Japan
基金
日本科学技术振兴机构;
关键词
TGIF2; amplification; overexpression; 20q11.2-12; ovarian cancer;
D O I
10.1006/bbrc.2000.3449
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homeodomain transcription factors play important roles in directing cellular proliferation and differentiation. A TALE-superclass homeodomain protein, multifunctional repressor of TGF beta-induced transcription. Here we report identification of TGIF2, a novel TALE-superclass homeodomain protein that shows distinct homology with TGIF, especially in its DNA-binding domain. TGIF2 is expressed ubiquitously in human tissues, with the highest levels being found in heart, kidney, and testis. The TGIF2 product contains a putative nuclear localization signal; translocation of the protein to the nucleus was confirmed by transfection of epitope-tagged cDNA, TGIF2 lies on chromosome 20q11.2-12. Since amplification of 20q is often observed among ovarian cancers, we determined the status of DNA copy-number and expression of TGIF2 in 14 ovarian-cancer cell lines, This gene was overexpressed in all lines that showed amplification by FISH analysis. The results suggested that TGIF2 may play an important role in the development and/or progression of some ovarian tumors through a mechanism of gene amplification. (C) 2000 Academic Press.
引用
收藏
页码:264 / 270
页数:7
相关论文
共 25 条
[1]   A novel homeobox protein which recognizes a TGT core and functionally interferes with a retinoid-responsive motif [J].
Bertolino, E ;
Reimund, B ;
WildtPerinic, D ;
Clerc, RG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (52) :31178-31188
[2]   Members of the Meis1 and Pbx homeodomain protein families cooperatively bind a cAMP-responsive sequence (CRS1) from bovine CYP17 [J].
Bischof, LJ ;
Kagawa, N ;
Moskow, JJ ;
Takahashi, Y ;
Iwamatsu, A ;
Buchberg, AM ;
Waterman, MR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (14) :7941-7948
[3]   Analysis of TALE superclass homeobox genes (MEIS, PBC, KNOX, Iroquois, TGIF) reveals a novel domain conserved between plants and animals [J].
Burglin, TR .
NUCLEIC ACIDS RESEARCH, 1997, 25 (21) :4173-4180
[4]   THE DNA-BINDING SPECIFICITY OF ULTRABITHORAX IS MODULATED BY COOPERATIVE INTERACTIONS WITH EXTRADENTICLE, ANOTHER HOMEOPROTEIN [J].
CHAN, SK ;
JAFFE, L ;
CAPOVILLA, M ;
BOTAS, J ;
MANN, RS .
CELL, 1994, 78 (04) :603-615
[5]   STRUCTURAL COMPLEXITY AND EVOLUTIONARY CONSERVATION OF THE DROSOPHILA HOMEOTIC GENE PROBOSCIPEDIA [J].
CRIBBS, DL ;
PULTZ, MA ;
JOHNSON, D ;
MAZZULLA, M ;
KAUFMAN, TC .
EMBO JOURNAL, 1992, 11 (04) :1437-1449
[6]  
FUKUDA Y, 2000, IN PRESS GENES CHROM
[7]   HOMEODOMAIN PROTEINS [J].
GEHRING, WJ ;
AFFOLTER, M ;
BURGLIN, T .
ANNUAL REVIEW OF BIOCHEMISTRY, 1994, 63 :487-526
[8]   HIGH-RESOLUTION CYTOGENETIC MAPPING OF 342 NEW COSMID MARKERS INCLUDING 43 RFLP MARKERS ON HUMAN CHROMOSOME-17 BY FLUORESCENCE IN-SITU HYBRIDIZATION [J].
INAZAWA, J ;
SAITO, H ;
ARIYAMA, T ;
ABE, T ;
NAKAMURA, Y .
GENOMICS, 1993, 17 (01) :153-162
[9]  
IWABUCHI H, 1995, CANCER RES, V55, P6172
[10]   A NEW HOMEOBOX GENE CONTRIBUTES THE DNA-BINDING DOMAIN OF THE T(1-19) TRANSLOCATION PROTEIN IN PRE-B ALL [J].
KAMPS, MP ;
MURRE, C ;
SUN, XH ;
BALTIMORE, D .
CELL, 1990, 60 (04) :547-555