Controlled cytokinin production in transgenic tobacco using a copper-inducible promoter

被引:86
作者
McKenzie, MJ
Mett, V
Reynolds, PHS
Jameson, PE
机构
[1] Massey Univ, Dept Plant Biol & Biotechnol, Coll Sci, Inst Mol Biosci, Palmerston North, New Zealand
[2] Univ Otago, Dept Bot, Dunedin, New Zealand
[3] Hort & Food Res Inst New Zealand Ltd, Plant Improvement Div, Palmerston North, New Zealand
关键词
D O I
10.1104/pp.116.3.969
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The cytokinin group of plant hormones regulates aspects of plant growth and development, including the release of lateral buds from apical dominance and the delay of senescence. In this work the native promoter of a cytokinin synthase gene (ipn was removed and replaced with a Cu-controllable promoter. Tobacco (Nicotiana tabacum L. cv tabacum) transformed with this Cu-inducible ipt gene (Cu-ipt) was morphologically identical to controls under noninductive conditions in almost all lines produced. However, three lines grew in an altered state, which is indicative of cytokinin overproduction and was confirmed by a full cytokinin analysis of one of these lines. The in vitro treatment of morphologically normal Cu-ipt transformants with Cu2+ resulted in delayed leaf senescence and an increase in cytokinin concentration in the one line analyzed. In vivo, inductive conditions resulted in a significant release of lateral buds from apical dominance. The morphological changes seen during these experiments may reflect the spatial aspect of control exerted by this gene expression system, namely expression from the root tissue only. These results confirmed that endogenous cytokinin concentrations in tobacco transformants can be temporally and spatially controlled by the induction of ipt gene expression through the Cu-controllable gene-expression system.
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收藏
页码:969 / 977
页数:9
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