Streptococcus pyogenes pili promote pharyngeal cell adhesion and biofilm formation

被引:186
作者
Manetti, Andrea G. O.
Zingaretti, Chiara
Falugi, Fabiana
Capo, Sabrina
Bombaci, Mauro
Bagnoli, Fabio
Gambellini, Gabriella
Bensi, Giuliano
Mora, Marirosa
Edwards, Andrew M.
Musser, James M.
Graviss, Edward A.
Telford, John L.
Grandi, Guido
Margarit, Immaculada
机构
[1] Novartis Vaccines & Diagnost, I-53100 Siena, Italy
[2] Univ Tuscia, Ctr Interdipartimentale Microscopia Eoettron, Viterbo, Italy
[3] Methodist Hosp, Ctr Mol & Translat Human Infect Dis Res, Houston, TX 77030 USA
[4] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA
关键词
D O I
10.1111/j.1365-2958.2007.05704.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Group A Streptococcus ( GAS, Streptococcus pyogenes) is a Gram-positive human pathogen responsible for several acute diseases and autoimmune sequelae that account for half a million deaths worldwide every year. GAS infections require the capacity of the pathogen to adhere to host tissues and assemble in cell aggregates. Furthermore, a role for biofilms in GAS pathogenesis has recently been proposed. Here we investigated the role of GAS pili in biofilm formation. We demonstrated that GAS pilus-negative mutants, in which the genes encoding either the pilus backbone structural protein or the sortase C1 have been deleted, showed an impaired capacity to attach to a pharyngeal cell line. The same mutants were much less efficient in forming cellular aggregates in liquid culture and microcolonies on human cells. Furthermore, mutant strains were incapable of producing the typical three-dimensional layer with bacterial microcolonies embedded in a carbohydrate polymeric matrix. Complemented mutants had an adhesion and aggregation phenotype similar to the wild-type strain. Finally, in vivo expression of pili was indirectly confirmed by demonstrating that most of the sera from human patients affected by GAS-mediated pharyngitis recognized recombinant pili proteins. These data support the role of pili in GAS adherence and colonization and suggest a general role of pili in all pathogenic streptococci.
引用
收藏
页码:968 / 983
页数:16
相关论文
共 45 条
[1]   Pili mediate specific adhesion of Streptococcus pyogenes to human tonsil and skin [J].
Abbot, Emily L. ;
Smith, Wendy D. ;
Siou, Gerard P. S. ;
Chiriboga, Carlos ;
Smith, Rebecca J. ;
Wilson, Janet A. ;
Hirst, Barry H. ;
Kehoe, Michael A. .
CELLULAR MICROBIOLOGY, 2007, 9 (07) :1822-1833
[2]   Assessment of Streptococcus pyogenes microcolony formation in infected skin by confocal laser scanning microscopy [J].
Akiyama, H ;
Morizane, S ;
Yamasaki, O ;
Oono, T ;
Iwatsuki, K .
JOURNAL OF DERMATOLOGICAL SCIENCE, 2003, 32 (03) :193-199
[3]   Therapeutic failures of antibiotics used to treat macrolide-susceptible streptococcus pyogenes infections may be due to biofilm formation [J].
Baldassarri, Lucilla ;
Creti, Roberta ;
Recchia, Simona ;
Imperi, Monica ;
Facinelli, Bruna ;
Giovanetti, Eleonora ;
Pataracchia, Marco ;
Alfarone, Giovanna ;
Orefici, Graziella .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (08) :2721-2727
[4]   Use of Lactococcus lactis expressing pili from group B Streptococcus as a broad-coverage vaccine against streptococcal disease [J].
Buccato, Scilla ;
Maione, Domenico ;
Rinaudo, Cira Daniela ;
Volpini, Gianfranco ;
Taddei, Anna Rita ;
Rosini, Roberto ;
Telford, John L. ;
Grandi, Guido ;
Margarit, Immaculada .
JOURNAL OF INFECTIOUS DISEASES, 2006, 194 (03) :331-340
[5]  
CAPARON MG, 1991, METHOD ENZYMOL, V204, P556
[6]   The mannose-sensitive hemagglutinin of Vibrio cholerae promotes adherence to zooplankton [J].
Chiavelli, DA ;
Marsh, JW ;
Taylor, RK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (07) :3220-3225
[7]   Patterns of virulence gene expression differ between biofilm and tissue communities of Streptococcus pyogenes [J].
Cho, KH ;
Caparon, MG .
MOLECULAR MICROBIOLOGY, 2005, 57 (06) :1545-1556
[8]   Bacterial biofilms: A common cause of persistent infections [J].
Costerton, JW ;
Stewart, PS ;
Greenberg, EP .
SCIENCE, 1999, 284 (5418) :1318-1322
[9]   Molecular mechanisms of adhesion, colonization, and invasion of group A streptococci [J].
Courtney, HS ;
Hasty, DL ;
Dale, JB .
ANNALS OF MEDICINE, 2002, 34 (02) :77-87
[10]   Pathogenesis of group A streptococcal infections [J].
Cunningham, MW .
CLINICAL MICROBIOLOGY REVIEWS, 2000, 13 (03) :470-+