Uptake and gene expression of naked plasmid DNA in cultured brain microvessel endothelial cells

被引：30

作者：

Nakamura, M ^{[1
]}

Davila-Zavala, P ^{[1
]}

Tokuda, H ^{[1
]}

Takakura, Y ^{[1
]}

Hashida, M ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Drug Discovery Res, Sakyo Ku, Kyoto 6068501, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 245卷 / 01期

关键词：

D O I：

10.1006/bbrc.1998.8334

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cellular uptake and gene expression of plasmid DNA and its cationic liposome complexes were studied using primary cultures of bovine brain microvessel endothelial cells (BMEC) developed as an in vitro model of the blood-brain barrier. An avid association of naked plasmid DNA with the BMEC monolayer was observed at 37 degrees C, which is comparable to that of the DNA/liposome complex,The cellular association significantly decreased at low temperature (4 degrees C), The binding at 4 degrees C was saturable and significantly inhibited by polyanions involving polyinosinic acid and dextran sulfate, typical ligands for the macrophage scavenger receptors, but not by polycytidylic acid or in the presence of EDTA, Unexpectedly, a significant gene expression in the BMEC was obtained by transfection with naked plasmid DNA although the expression level was lower than that obtained by plasmid DNA/cationic liposome complex. Taken together, cultured capillary endothelial cells derived from the brain are able to take up naked plasmid DNA via a scavenger receptor like-mediated mechanism for polyanions and gene expression in the cells takes place. (C) 1998 Academic Press.

引用

页码：235 / 239

页数：5

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