The solution structure and dynamics of the pleckstrin homology domain of G protein-coupled receptor kinase 2 (β-adrenergic receptor kinase 1) -: A binding partner of Gβγ subunits

被引:66
作者
Fushman, D
Najmabadi-Haske, T
Cahill, S
Zheng, J
LeVine, H
Cowburn, D
机构
[1] Rockefeller Univ, Lab Phys Biochem, New York, NY 10021 USA
[2] Warner Lambert Parke Davis, Parke Davis Labs, Ann Arbor, MI 48105 USA
关键词
D O I
10.1074/jbc.273.5.2835
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The solution structure of an extended pleckstrin homology (PII) domain from the beta-adrenergic receptor kinase is obtained by high resolution NMR. The structure establishes that the beta-adrenergic receptor kinase extended PH domain has the same fold and topology as other PH domains, and there are several unique features, most notably an extended C terminal alpha-helix that behaves as a molten helix, and a surface charge polarity that is extensively modified by positive residues in the extended alpha-helix and the C terminus. These observations complement biochemical evidence that the C-terminal portion of this PH domain participates in protein-protein interactions with G(beta gamma) subunits. This suggests that the C-terminal segment of the PH domain may function to mediate protein-protein interactions with the targets of PH domains.
引用
收藏
页码:2835 / 2843
页数:9
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