A novel form of gonadotropin-releasing hormone in the medaka, Oryzias latipes

被引：89

作者：

Okubo, K

Amano, M

Yoshiura, Y

Suetake, H

Aida, K ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Aquat Biosci, Bunkyo Ku, Tokyo 1138657, Japan

[2] Kitasato Univ, Sch Fisheries Sci, Sanriku, Iwate 0220101, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 276卷 / 01期

关键词：

gonadotropin-releasing hormone (GnRH); molecular forms; mdGnRH; cGnRH-II; sGnRH; phylogeny; gene; expression; medaka;

D O I：

10.1006/bbrc.2000.3476

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study has identified three molecular forms of gonadotropin-releasing hormone (GnRH) in the brain of a teleost, the medaka, by isolation of their cDNAs, This species has a novel GnRH, which is here named medaka-type GnRH (mdGnRH), in addition to two characterized forms, chicken-II-type GnRH (cGnRH-II) and salmon-type GnRH (sGnRH), Phylogenetic analysis showed that mdGnRH is a medaka homolog of and seabream-type GnRH (sbGnRH) and mammalian-type GnRH (mGnRH) in other species, and suggested that all vertebrates have three distinct GnRHs, Furthermore, in situ hybridization revealed that the mdGnRH gene is expressed only in neurons clustered within the preoptic area as sbGnRH and mGnRH genes in other species are, while the genes for cGnRH-II and sGnRH are only in the midbrain tegmentum and nucleus olfactoretinalis, respectively. This result suggested that mdGnRH is a hypophysiotropic factor and the other two forms are involved in other physiological events as neuromodulators or neurotransmitters. (C) 2000 Academic Press.

引用

页码：298 / 303

页数：6

共 22 条

[1] ACTIVATION OF SALMON GONADOTROPIN-RELEASING-HORMONE SYNTHESIS BY 17-ALPHA-METHYLTESTOSTERONE ADMINISTRATION IN YEARLING MASU SALMON, ONCORHYNCHUS-MASOU [J].