Sequential increases in capillarization and mitochondrial enzymes in low-frequency-stimulated rabbit muscle

被引:70
作者
Skorjanc, D [1 ]
Jaschinski, F [1 ]
Heine, G [1 ]
Pette, D [1 ]
机构
[1] Univ Konstanz, Fac Biol, D-78457 Constance, Germany
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1998年 / 274卷 / 03期
关键词
electrical stimulation; mitochondrial enzyme activity; morphometry; vascular endothelial growth factor;
D O I
10.1152/ajpcell.1998.274.3.C810
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To investigate temporal changes in capillarization and increases in mitochondrial enzyme activity, rabbit tibialis anterior muscles underwent chronic low-frequency stimulation for up to 50 days. Capillary density (CD), capillaty-to-fiber ratio (C/F), intercapillary distance (ICD), and mean capillary area (MCA), as well as several other parameters of capillarization, were examined. In addition, tissue levels of mRNA specific to vascular endothelial growth factor (VEGF) were assessed by reverse transcriptase-polymerase chain reaction. Citrate synthase (CS) activity, a marker of aerobic-oxidative metabolic potential. was measured in the same muscles. Significant increases in CD and C/F. respectively, and decreases in ICD and MCA were observed after 2 days. These changes reached stable maxima by 14 days. The increases in capillarization occurred in a fiber-type-specific manner, affecting type IId fibers before types IIda and IIa. VEGF mRNA levels increased in a bimodal time pattern with a first elevation (2.5-fold) after 1 day and a second (9-fold) after 6-8 days. Increases in CS were first noted after 8 days. Obviously, increases in capillarization as induced by enhanced contractile activity precede increases in the aerobic-oxidative potential of energy metabolism.
引用
收藏
页码:C810 / C818
页数:9
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