The role of the FtsH and Deg proteases in the repair of UV-B radiation-damaged photosystem II in the cyanobacterium Synechocystis PCC 6803

被引:35
作者
Cheregi, Otilia
Sicora, Cosmin
Kos, Peter B.
Barker, Myles
Nixon, Peter J.
Vass, Imre [1 ]
机构
[1] Biol Res Ctr, Inst Plant Biol, H-6701 Szeged, Hungary
[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol, London SW7 2AZ, England
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2007年 / 1767卷 / 06期
基金
英国生物技术与生命科学研究理事会;
关键词
photosystem II; D1-protein; D2-protein; FtsH protease; UV-B damage;
D O I
10.1016/j.bbabio.2006.11.016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The photosystem two (PSII) complex found in oxygenic photosynthetic organisms is susceptible to damage by UV-B irradiation and undergoes repair in vivo to maintain activity. Until now there has been little information on the identity of the enzymes involved in repair. In the present study we have investigated the involvement of the FtsH and Deg protease families in the degradation of UV-B-damaged PSII reaction center subunits, D1 and D2, in the cyanobacterium Synechocystis 6803. PSII activity in a Delta FtsH (slr0228) strain, with an inactivated slr0228 gene, showed increased sensitivity to UV-B radiation and impaired recovery of activity in visible light after UV-B exposure. In contrast, in Delta Deg-G cells, in which all the three deg genes were inactivated, the damage and recovery kinetics were the same as in the WT. Immunoblotting showed that the loss of both the D1 and D2 proteins was retarded in A FtsH (slr0228) during UV-B exposure, and the extent of their restoration during the recovery period was decreased relative to the WT. However, in the Delta Deg-G cells the damage and recovery kinetics of D1 and D2 were the same as in the WT. These data demonstrate a key role of FtsH (slr0228), but not the Deg proteases, for the repair of PSII during and following UV-B radiation at the step of degrading both of the UV-B damaged D1 and D2 reaction center subunits. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:820 / 828
页数:9
相关论文
共 46 条
[1]  
[Anonymous], HDB PHOTOSYNTHESIS
[2]   PHOTOINHIBITION OF PHOTOSYSTEM-2 - INACTIVATION, PROTEIN DAMAGE AND TURNOVER [J].
ARO, EM ;
VIRGIN, I ;
ANDERSSON, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1143 (02) :113-134
[3]   A critical role for the Var2 FtsH homologue of Arabidopsis thaliana in the photosystem II repair cycle in vivo [J].
Bailey, S ;
Thompson, E ;
Nixon, PJ ;
Horton, P ;
Mullineaux, CW ;
Robinson, C ;
Mann, NH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (03) :2006-2011
[4]  
BARKER M, 2006, J BIOL CHEM, V281, P1
[5]   Role of visible light in the recovery of photosystem II structure and function from ultraviolet-B stress in higher plants [J].
Bergo, E ;
Segalla, A ;
Giacometti, GM ;
Tarantino, D ;
Soave, C ;
Andreucci, F ;
Barbato, R .
JOURNAL OF EXPERIMENTAL BOTANY, 2003, 54 (388) :1665-1673
[6]   Cyclic nucleotides, the photosynthetic apparatus and response to a UV-B stress in the cyanobacterium Synechocystis sp PCC 6803 [J].
Cadoret, JC ;
Rousseau, B ;
Perewoska, I ;
Sicora, C ;
Cheregi, O ;
Vass, I ;
Houmard, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (40) :33935-33944
[7]   Architecture of the photosynthetic oxygen-evolving center [J].
Ferreira, KN ;
Iverson, TM ;
Maghlaoui, K ;
Barber, J ;
Iwata, S .
SCIENCE, 2004, 303 (5665) :1831-1838
[8]   DEGRADATION OF D2 PROTEIN DUE TO UV-B IRRADIATION OF THE REACTION-CENTER OF PHOTOSYSTEM-II [J].
FRISO, G ;
BARBATO, R ;
GIACOMETTI, GM ;
BARBER, J .
FEBS LETTERS, 1994, 339 (03) :217-221
[9]   DEGRADATION OF PHOTOSYSTEM-II REACTION-CENTER D1-PROTEIN INDUCED BY UVB RADIATION IN ISOLATED THYLAKOIDS - IDENTIFICATION AND CHARACTERIZATION OF C-TERMINAL AND N-TERMINAL BREAKDOWN PRODUCTS [J].
FRISO, G ;
SPETEA, C ;
GIACOMETTI, GM ;
VASS, I ;
BARBATO, R .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1994, 1184 (01) :78-84
[10]   SEPARATE PHOTOSENSITIZERS MEDIATE DEGRADATION OF THE 32-KDA PHOTOSYSTEM-II REACTION CENTER PROTEIN IN THE VISIBLE AND UV SPECTRAL REGIONS [J].
GREENBERG, BM ;
GABA, V ;
CANAANI, O ;
MALKIN, S ;
MATTOO, AK ;
EDELMAN, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) :6617-6620