Cloning of a functional promoter of the human sodium/iodide-symporter gene

被引：48

作者：

Behr, M ^{[1
]}

Schmitt, TL ^{[1
]}

Espinoza, CR ^{[1
]}

Loos, U ^{[1
]}

机构：

[1] Univ Ulm Klinikum, Innere Med Abt 1, D-89081 Ulm, Germany

来源：

BIOCHEMICAL JOURNAL | 1998年 / 331卷

关键词：

D O I：

10.1042/bj3310359

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced genomic DNA from a human library extending 1300 bp upstream the 5'-untranslated sequence of the cDNA coding for the sodium/iodide symporter. In transient transfection assays this sequence exhibited promoter activity, which could be confined to nucleotides -443 to -395 relative to the ATG start codon. This minimal promoter, including a putative GC-and TATA-box, was preferentially activated in the rat thyroid cell line FRTL-5, but was also active in non-thyroidal cells, such as COS-7 and Chinese-hamster ovary, albeit to a markedly lower extent.

引用

页码：359 / 363

页数：5