Trichostatin A induces myocardial differentiation of monkey ES cells

被引：42

作者：

Hosseinkhani, Mohsen

Hasegawa, Koji

Ono, Koh

Kawamura, Teruhisa

Takaya, Tomohide

Morimoto, Tatsuya

Wada, Hiromichi

Shimatsu, Akira

Prat, Susana Gonzalez

Suemori, Hirofumi

Nakatsuji, Norio

Kita, Toru

机构：

[1] Natl Hosp Org, Kyoto Med Ctr, Clin Res Inst, Div Translat Res,Fushimi Ku, Kyoto 6128555, Japan

[2] Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Kyoto, Japan

[3] Kyoto Univ, Inst Frontier Med Sci, Kyoto, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 356卷 / 02期

关键词：

primate embryonic stem cell; myocardial differentiation; histone acetylation;

D O I：

10.1016/j.bbrc.2007.02.151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human embryonic stem (ES) cell lines are one of the possible sources of cardiac myocytes to be transplanted in patients with end-staged heart failure. However, prior to the application of human of ES cells for heart failure therapy, it is critical to validate their clinical use in large animals such as primates. Cynomolgus monkey ES cells have similar properties to human ES cells and can be used for primate studies. We demonstrate that 24-h stimulation by a histone deacetylase inhibitor, trichostatin A (TSA) facilitated myocardial differentiation of monkey ES cells with embryonic bodies that were seeded on gelatin-coated dishes. TSA-induced acetylating of histone-3/4 and expression of p300, one of the intrinsic histone acetyltransferases. Thus, such induction as well as inhibition of histone deacetylase may be involved in TSA-induced differentiation of cynomolgus monkey ES cells into cardiomyocytes. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：386 / 391

页数：6

共 24 条

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