Control of human thymocyte migration by Neuropilin-1/Semaphorin-3A-mediated interactions

被引:90
作者
Lepelletier, Yves
Smaniotto, Salete
Hadj-Slimane, Reda
Villa-Verde, Dea Maria Serra
Nogueira, Ana Cristina
Dardenne, Mireille
Hermine, Olivier
Savino, Wilson
机构
[1] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Dept Immunol, Lab Thymus Res, Rio De Janeiro, Brazil
[2] Univ Paris 05, UMR 8147, CNRS, F-75743 Paris, France
[3] Univ Fed Alagoas, Dept Morphol, BR-57072970 Maceio, Brazil
[4] Univ Paris 05, Unite 648, Inst Natl Sante & Rech Med, F-75006 Paris, France
[5] TRAGEX Pharma, F-75015 Paris, France
关键词
extracellular matrix; integrins; thymocyte adhesion and migration;
D O I
10.1073/pnas.0700705104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
It is largely established that molecules first discovered in the nervous system are also found in the immune system. Neuropilin-1 (NP-1) was initially identified to mediate semaphorin-induced chemorepulsion during brain development and is also involved in peripheral T cell/dendritic cell interactions. Herein, we studied NP-1 during T cell development in the human thymus. NP-1 is expressed in both cortex and medulla of thymic lobules, being found in distinct CD4/CD8-defined thymocyte subsets. NP-1 is also found in thymic epithelial cells (TEC) in situ and in vitro, and is recruited at the site of TEC-thymocyte contact. Moreover, NP-1 was rapidly up-regulated during thymocyte stimulation by T cell receptor (TCR) and IL-7 or after adhesion to TEC. Semaphorin-3A (Sema-3A), a natural ligand of NP-1, is also present in human thymus, both in TEC and thymocytes, being up-regulated in thymocytes after TCR engagement. Functionally, Sema-3A decreases the adhesion capacity of NP-1(+) thymocytes and induces their migration by a repulsive effect. In conclusion, we show here that NP-1/Sema-3A-mediated interactions participate in the control of human thymocyte development.
引用
收藏
页码:5545 / 5550
页数:6
相关论文
共 28 条
[1]   Chemokines and lymphopoiesis in human thymus [J].
Annunziato, F ;
Romagnani, P ;
Cosmi, L ;
Lazzeri, E ;
Romagnani, S .
TRENDS IN IMMUNOLOGY, 2001, 22 (05) :277-281
[2]   Chemokines in lymphopoiesis and lymphoid organ development [J].
Ansel, KM ;
Cyster, JG .
CURRENT OPINION IN IMMUNOLOGY, 2001, 13 (02) :172-179
[3]   Phagocytic cells of the thymic reticulum interact with thymocytes via extracellular matrix ligands and receptors [J].
Ayres-Martins, S ;
Lannes-Vieira, J ;
Farias-de-Oliveira, DA ;
Brito, JM ;
Villa-Verde, DMS ;
Savino, W .
CELLULAR IMMUNOLOGY, 2004, 229 (01) :21-30
[4]   Human thymus contains IFN-α-producing CD11c-, myeloid CD11c+ and mature interdigitating dendritic cells [J].
Bendriss-Vermare, N ;
Barthélémy, C ;
Durand, I ;
Bruand, C ;
Dezutter-Dambuyant, C ;
Moulian, N ;
Berrih-Aknin, S ;
Caux, C ;
Trinchieri, G ;
Brière, F .
JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (07) :835-844
[5]   Impaired migration of NOD mouse thymocytes:: a fibronectin receptor-related defect [J].
Cotta-De-Almeida, V ;
Villa-Verde, DMS ;
Lepault, F ;
Pléau, JM ;
Dardenne, M ;
Savino, W .
EUROPEAN JOURNAL OF IMMUNOLOGY, 2004, 34 (06) :1578-1587
[6]   Chemorepulsion and thymocyte emigration [J].
Cyster, JG .
JOURNAL OF CLINICAL INVESTIGATION, 2002, 109 (08) :1011-1012
[7]   THE ORGANIZATION OF F-ACTIN AND MICROTUBULES IN GROWTH CONES EXPOSED TO A BRAIN-DERIVED COLLAPSING FACTOR [J].
FAN, JH ;
MANSFIELD, SG ;
REDMOND, T ;
GORDONWEEKS, PR ;
RAPER, JA .
JOURNAL OF CELL BIOLOGY, 1993, 121 (04) :867-878
[8]  
FERNANDEZ E, 1994, BLOOD, V83, P3245
[9]   Semaphorin3A enhances endocytosis at sites of receptor-F-actin colocalization during growth cone collapse [J].
Fournier, AE ;
Nakamura, F ;
Kawamoto, S ;
Goshima, Y ;
Kalb, RG ;
Strittmatter, SM .
JOURNAL OF CELL BIOLOGY, 2000, 149 (02) :411-421
[10]  
Guyden JC, 2003, INT REV CYTOL, V223, P1