Differential activity of nitric oxide synthase in human nasal mucosa and polyps

被引:55
作者
Ramis, I [1 ]
Lorente, J [1 ]
RoselloCatafau, J [1 ]
Quesada, P [1 ]
Gelpi, E [1 ]
机构
[1] HOSP GEN UNIV VALLE HEBRON,DEPT OTORHINOLARYNGOL,BARCELONA,SPAIN
关键词
nasal mucosa; nasal polyposis; NADPH-diaphorase; nitric oxide synthase;
D O I
10.1183/09031936.96.09020202
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Nitric oxide (NO) plays an important regulatory role in airway function and seems to be implicated in the pathophysiology of several airway diseases. To better understand the involvement of NO in the upper airways, we examined the presence of nitric oxide synthase (NOS) activity in human nasal mucosa and nasal polyp tissues. Nasal mucosa was obtained from seven patients undergoing septoplasty, and nasal polyps came from nine patients following polypectomy, NOS activity was quantified in tissue homogenates using the citrulline release assay and localized in tissue sections using reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry. The results showed that nasal polyps (n=9) contained higher levels of total NOS activity (mean+/-SD 5.94+/-5.71, range 1.29-18.0 pmol . min(-1). mg protein) than nasal mucosa tissues (n=7) (0.28+/-0.22, range 0.01-057 pmol . min(-1). mg protein). In addition, nasal polyps mainly contained inducible NOS activity (4.67+/-4.57, range 1.25-15.5 pmol . min(-1). mg protein) whereas in nasal mucosa all NOS activity detected was in constitutive form. In both cases, NOS activity was localized in the epithelial cells. Since NO synthase is induced in inflamed upper airways, we conclude that NO may be an important inflammatory mediator in the respiratory system and that the epithelium may be a source of NO production in the human upper airways.
引用
收藏
页码:202 / 206
页数:5
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