A β-D-glucan from the sclerotia of Pleurotus tuber-regium (Fr.) Sing

被引:34
作者
Deng, CH [1 ]
Yang, XL [1 ]
Gu, XM [1 ]
Wang, Y [1 ]
Zhou, JY [1 ]
Xu, HB [1 ]
机构
[1] Huazhong Univ Sci & Technol, Dept Chem, Wuhan 430074, Peoples R China
关键词
Pleurotus tuber-regium; polysaccharide; structural studies; (1 -> 3)-beta-D-glucan;
D O I
10.1016/S0008-6215(00)00140-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An alkali-soluble polysaccharide (Hunai polysaccharide, 1) from the fruit body of Pleurotus tuber-regium (Fr.) Sing was shown to be homogeneous by gel permeation chromatography and its molecular weight was similar to 4.3 x 10(5). Complete acid hydrolysis, periodate oxidation, Smith degradation, methylation, FT-IR and C-13 NMR analysis, complex formation with Congo Red, indicated that 1 has a beta-(1-->3)-linked D-glucopyranosyl backbone with a single beta-D-glucopyranosyl group at O-6 of every third glucose residue. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:629 / 633
页数:5
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