Novel phosphoramidite monomer for the site-selective incorporation of a diastereochemically pure phosphoramidate to oligonucleotide

被引:14
作者
Endo, M [1 ]
Komiyama, M [1 ]
机构
[1] UNIV TOKYO,GRAD SCH ENGN,DEPT CHEM & BIOTECHNOL,BUNKYO KU,TOKYO 113,JAPAN
关键词
D O I
10.1021/jo951621r
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
Diastereochemically pure dithymidine phosphoramidates have been site-selectively incorporated into synthetic oligonucleotides by a phosphoramidite technique. By using the terminal amino residues bound to the chiral phosphoramidates, various functional residues have been attached to the oligonucleotides in stereospecific ways. No racemization takes place during these procedures. The dependence of the duplex- and tripler-forming activities of these tethered and functionalized oligonucleotides on the diastereochemistry of the phosphoramidate is shown.
引用
收藏
页码:1994 / 2000
页数:7
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