The antiviral protein viperin is a radical SAM enzyme

被引：103

作者：

Duschene, Kaitlin S.

Broderick, Joan B. ^{[1
]}

机构：

[1] Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 USA

来源：

FEBS LETTERS | 2010年 / 584卷 / 06期

关键词：

Viperin; Antiviral; Radical SAM; Iron-sulfur cluster; Metalloenzyme; S-Adenosylmethionine; LYASE-ACTIVATING ENZYME; IRON-SULFUR CLUSTER; GENE-EXPRESSION; BIOTIN SYNTHASE; INFLUENZA-VIRUS; LIPID RAFTS; ADENOSYLMETHIONINE; SUPERFAMILY; BINDING; IDENTIFICATION;

D O I：

10.1016/j.febslet.2010.02.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Viperin, an interferon-inducible antiviral protein, is shown to bind an iron-sulfur cluster, based on iron analysis as well as UV-Vis and electron paramagnetic resonance spectroscopic data. The reduced protein contains a [4Fe-4S](1+) cluster whose g-values are altered upon addition of S-adenosylmethionine (SAM), consistent with SAM coordination to the cluster. Incubation of reduced viperin with SAM results in reductive cleavage of SAM to produce 5'-deoxyadenosine (5'-dAdo), a reaction characteristic of the radical SAM superfamily. The 5'-dAdo cleavage product was identified by a combination of HPLC and mass spectrometry analysis. (C) 2010 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：1263 / 1267

页数：5

共 29 条

[1] ASHBY MN, 1989, J BIOL CHEM, V264, P635
[2] Anaerobic functionalization of unactivated C-H bonds
Booker, Squire J.
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2009, 13 (01) : 58 - 73
[3] Vesicular stomatitis virus and pseudorabies virus induce a vig1/cig5 homologue in mouse dendritic cells via different pathways
Boudinot, P
Riffault, S
Salhi, S
Carrat, C
Sedlik, C
Mahmoudi, N
Charley, B
Benmansour, A
[J]. JOURNAL OF GENERAL VIROLOGY, 2000, 81 : 2675 - 2682
[4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5] Pyruvate formate-lyase-activating enzyme:: Strictly anaerobic isolation yields active enzyme containing a [3Fe-4S]+ cluster
Broderick, JB
Henshaw, TF
Cheek, J
Wojtuszewski, K
Smith, SR
Trojan, MR
McGhan, RM
Kopf, A
Kibbey, M
Broderick, WE
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 269 (02) : 451 - 456
[6] Viperin (cig5), an IFN-inducible antiviral protein directly induced by human cytomegalovirus
Chin, KC
Cresswell, P
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (26) : 15125 - 15130
[7] RAPID COLORIMETRIC MICROMETHOD FOR THE QUANTITATION OF COMPLEXED IRON IN BIOLOGICAL SAMPLES
FISH, WW
[J]. METHODS IN ENZYMOLOGY, 1988, 158 : 357 - 364
[8] Frey PA, 2008, CRIT REV BIOCHEM MOL, V43, P63, DOI [10.1080/10409230701829169, 10.1080/10409230701829169 ]
[9] SYNTHETIC ROUTES TO FE2S2, FE3S4, FE4S4, AND FE6S9 CLUSTERS FROM THE COMMON PRECURSOR [FE(SC2H5)4]2- - STRUCTURES AND PROPERTIES OF [FE3S4(SR)4]3- AND [FE6S9(SC2H5)2]4-, EXAMPLES OF THE NEWEST TYPES OF FE-S-SR CLUSTERS
HAGEN, KS
WATSON, AD
HOLM, RH
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (12) : 3905 - 3913
[10] Analysis of ISG expression in chronic hepatitis C identifies viperin as a potential antiviral effector
Helbig, KJ
Lau, DTY
Semendric, L
Harley, HAJ
Beard, MR
[J]. HEPATOLOGY, 2005, 42 (03) : 702 - 710

← 1 2 3 →