Peptide-Modified "Smart" Hydrogels and Genetically Engineered Stem Cells for Skeletal Tissue Engineering

被引：40

作者：

Garty, Shai ^{[1
]}

Kimelman-Bleich, Nadav ^{[2
]}

Hayouka, Zvi ^{[3
]}

Cohn, Daniel ^{[1
]}

Friedler, Assaf ^{[3
]}

Pelled, Gadi ^{[2
,4
,5
]}

Gazit, Dan ^{[2
,4
,5
]}

机构：

[1] Hebrew Univ Jerusalem, Casali Inst Appl Chem, Inst Chem, IL-91904 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Skeletal Biotech Lab, Fac Med Dent, IL-91120 Jerusalem, Israel

[3] Hebrew Univ Jerusalem, Inst Chem, Dept Organ Chem, IL-91904 Jerusalem, Israel

[4] Cedars Sinai Med Ctr, Dept Surg, Los Angeles, CA 90048 USA

[5] Cedars Sinai Med Ctr, CS RMI, Los Angeles, CA 90048 USA

来源：

BIOMACROMOLECULES | 2010年 / 11卷 / 06期

基金：

美国国家卫生研究院;

关键词：

REGIONAL GENE-THERAPY; BONE-MARROW-CELLS; BLOCK-COPOLYMERS; ELECTRICAL-CONDUCTIVITY; SELF-DIFFUSION; SPINAL-FUSION; DELIVERY; SCAFFOLDS; CARTILAGE; MATRIX;

D O I：

10.1021/bm100157s

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stimuli responsive or "smart" hydrogels are of interest for tissue-engineering applications, featuring the advantages of minimally invasive application. Currently, these materials have yet to be used as a biological replacement in restoring the function of damaged tissues or organs. The aim of this study was to demonstrate the advantages of thermoresponsive, peptide-containing hydrogels as a supportive matrix for genetically engineered stem cells. We used injectable hydrogels, enabling cell delivery to the desired site and providing adequate scaffolding postimplantation. Thermoresponsive hydrogels were developed based on amphiphilic block copolymers of polyethylene-oxide and polypropylene-oxide end-capped with methacrylate or maleimide entities and further reacted with RGD-containing peptides. Cell metabolic activity and survival within those hydrogels was studied, illustrating that the stable peptide polymer conjugate is required for prolonged cell support. The unique polymer characteristics, combined with its enhanced cell interactions, suggest the potential use of these biomaterials in various tissue engineering applications.

引用

页码：1516 / 1526

页数：11

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