Two point mutations identified in emmer wheat generate null Wx-A1 alleles

被引:25
作者
Saito, M [1 ]
Nakamura, T [1 ]
机构
[1] Tohoku Natl Agr Res Ctr, Dept Crop Breeding, Morioka, Iwate 0200198, Japan
关键词
D O I
10.1007/s00122-004-1830-6
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
In this report, the Wx-A1 mutations carried by a Triticum dicoccoides line from Israel and a Triticum dicoccum line from Yugoslavia are characterized. A single nucleotide insertion in the T. dicoccoides null allele and a single nucleotide deletion in the T. dicoccum null allele each cause frameshift mutations that induce premature termination codons more than 55 nucleotides upstream of the last exon-exon junction. In both mutants, Wx-A1 transcripts were detectable in 10 day post-anthesis endosperm by relative RT-PCR. However, transcript levels of the T. dicoccoides and T. dicoccum null alleles were reduced to approximately 6.5 and 1.5% of wild-type, respectively. Therefore, the lack of Wx-A1 protein in the mutants appears to be largely due to nonsense-mediated mRNA decay. The two mutations described here arose independently, and are not related to either of the Wx-A1 mutations identified in common wheat.
引用
收藏
页码:276 / 282
页数:7
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