Autoantibodies to human recombinant erythropoietin in patients with systemic lupus erythematosus - Correlation with anemia

被引:58
作者
Tzioufas, AG [1 ]
Kokori, SI [1 ]
Petrovas, CI [1 ]
Moutsopoulos, HM [1 ]
机构
[1] Univ Athens, GR-10679 Athens, Greece
来源
ARTHRITIS AND RHEUMATISM | 1997年 / 40卷 / 12期
关键词
D O I
10.1002/art.1780401216
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. To investigate the existence of circulating autoantibodies to erythropoietin (EPO) in sera from patients with systemic lupus erythematosus (SLE), and to correlate their presence with anemia and clinical activity. Methods. Ninety-two consecutive patients with SLE, 80 patients with rheumatoid arthritis, and 42 normal individuals were studied, The patients with SLE were categorized into 3 groups according to hemoglobin (Hgb) level: group A (45 patients with Hgb > 12 gm/dl), group B (26 patients with Hgb 10.1-12 gm/dl), and group C (21 patients with Hgb less than or equal to 10 gm/dl), In all patients-with SLE, the disease activity was evaluated using the European Consensus Lupus Activity Measurement scale. Antibodies to EPO were detected using an enzyme-linked immunosorbent assay and purified recombinant human EPO as antigen, The specificity of the method was evaluated with homologous and crossreactive inhibition assays. Results. Antibodies to EPO were found in 15.2% of the SLE patient sera, The distribution of these antibodies among the 3 groups of SLE patients was as follows: 8.8% (4 of 45) from group A, 15.4% (4 Of 26) from group B, and 28.6% (6 of 21) from group C, The prevalence of antibodies to EPO in patients with severe anemia (group C) was statistically significantly higher compared with patients without anemia (chi(2) = 4.31, P < 0.05). Patients with antibodies to EPO had higher disease activity scores (P < 0.005) and lower levels of the C4 component of complement (P < 0.05) compared with patients without antibodies to EPO. Conclusion. In this study, the presence of antibodies to EPO in the sera of SLE patients is demonstrated for the first time, The presence of these antibodies is associated with severe anemia and active disease.
引用
收藏
页码:2212 / 2216
页数:5
相关论文
共 15 条
  • [1] THE AMERICAN-RHEUMATISM-ASSOCIATION 1987 REVISED CRITERIA FOR THE CLASSIFICATION OF RHEUMATOID-ARTHRITIS
    ARNETT, FC
    EDWORTHY, SM
    BLOCH, DA
    MCSHANE, DJ
    FRIES, JF
    COOPER, NS
    HEALEY, LA
    KAPLAN, SR
    LIANG, MH
    LUTHRA, HS
    MEDSGER, TA
    MITCHELL, DM
    NEUSTADT, DH
    PINALS, RS
    SCHALLER, JG
    SHARP, JT
    WILDER, RL
    HUNDER, GG
    [J]. ARTHRITIS AND RHEUMATISM, 1988, 31 (03): : 315 - 324
  • [2] BAILEY FA, 1989, ARTHRITIS RHEUM, V32, P901
  • [3] BENCIVELLI W, 1992, CLIN EXP RHEUMATOL, V10, P549
  • [4] BENDTZEN K, 1996, AUTOANTIBODIES
  • [5] Brief report: Autoantibodies against erythropoietin in a patient with pure red-cell aplasia
    Casadevall, N
    Dupuy, E
    MolhoSabatier, P
    Tobelem, G
    Varet, B
    Mayeux, P
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1996, 334 (10) : 630 - 633
  • [6] KRANTZ SB, 1991, BLOOD, V77, P419
  • [7] LIU H, 1995, CLIN EXP IMMUNOL, V100, P480
  • [8] MANOUSSAKIS MN, 1987, CLIN EXP IMMUNOL, V69, P557
  • [9] NITSCHE A, 1988, J RHEUMATOL, V15, P1012
  • [10] ANTIERYTHROPOIETIN ANTIBODIES IN HYPERVISCOSITY SYNDROME ASSOCIATED WITH GIANT LYMPH-NODE HYPERPLASIA (GLNH, CASTLEMANS DISEASE)
    STEINBERG, JJ
    HUANG, PL
    LJUBICH, P
    LEEHUANG, S
    [J]. BRITISH JOURNAL OF HAEMATOLOGY, 1990, 74 (04) : 543 - 544