Two tandemly repeated telomere-associated sequences in Nicotiana plumbaginifolia

被引：27

作者：

Chen, CM ^{[1
]}

Wang, CT

Wang, CJ

Ho, CH

Kao, YY

Chen, CC

机构：

[1] Acad Sinica, Inst Bot, Taipei 115, Taiwan

[2] Natl Taiwan Univ, Dept Bot, Taipei 10764, Taiwan

[3] Natl Taiwan Univ, Inst Biochem Sci, Taipei, Taiwan

来源：

CHROMOSOME RESEARCH | 1997年 / 5卷 / 08期

关键词：

fluorescence in situ hybridization; highly repeated tandem sequence; Nicotiana plumbaginifolia; telomere-associated sequence;

D O I：

10.1023/A:1018449920968

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two tandemly repeated telomere-associated sequences, NP3R and NP4R, have been isolated from Nicotiana plumbaginifolia. The length of a repeating unit for NP3R and NP4R is 165 and 180 nucleotides respectively. The abundance of NP3R, NP4R and telomeric repeats is, respectively, 8.4 x 10(4), 6 x 10(3) and 1.5 x 10(6) copies per haploid genome of N. plumbaginifolia. Fluorescence in situ hybridization revealed that NP3R is located at the ends and/or in interstitial regions of all 10 chromosomes and NP4R on the terminal regions of three chromosomes in the haploid genome of N. plumbaginifolia. Sequence homology search revealed that not only are NP3R and NP4R homologous to HRS60 and GRS, respectively, two tandem repeats isolated from N. tabacum, but that NP3R and NP4R are also related to each other, suggesting that they originated from a common ancestral sequence. The role of these repeated sequences in chromosome healing is discussed based on the observation that two to three copies of a telomere-similar sequence were present in each repeating unit of NP3R and NP4R.

引用

页码：561 / 568

页数：8

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