Expression of polycystin-1 C-terminal fragment enhances the ATP-induced Ca2+ release in human kidney cells

被引:25
作者
Aguiari, G
Campanella, M
Manzati, E
Pinton, P
Banzi, M
Moretti, S
Piva, R
Rizzuto, R
del Senno, L
机构
[1] Univ Ferrara, Sect Gen Pathol, Dept Biochem & Mol Biol, I-44100 Ferrara, Italy
[2] Univ Ferrara, Sect Gen Pathol, Dept Expt & Diagnost Med, I-44100 Ferrara, Italy
[3] Univ Ferrara, Sect Hematol, Dept Adv Biomed Sci & Therapies, I-44100 Ferrara, Italy
关键词
polycystin-1; polycystin-2; TrkPC1 chimeric receptors; epithelial cells; HEK; 293; cells; HeLa cells; protein expression; intracellular Ca2+;
D O I
10.1016/S0006-291X(02)03011-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Polycystin-1 (PC1) is a membrane protein expressed in tubular epithelia of developing kidneys and in other ductal structures. Recent studies indicate this protein to be putatively important in regulating intracellular Ca2+ levels in various cell types, but little evidence exists for kidney epithelial cells. Here we examined the role of the PC1 cytoplasmic tail on the activity of store operated Ca2+ channels in human kidney epithelial HEK-293 cell line. Cells were transiently transfected with chimeric proteins containing 1-226 or 26-226 aa of the PC1 cytoplasmic tail fused to the transmembrane domain of the human Trk-A receptor: TrkPC1 wild-type and control Trk truncated peptides were expressed at comparable levels and localized at the plasma membrane. Ca2+ measurements were performed in cells co-transfected with PC1 chimeras and the cytoplasmic Ca2+-sensitive photoprotein aequorin, upon activation of the phosphoinositide pathway by ATP, that, via purinoceptors, is coupled to the release of Ca2+ from intracellular stores. The expression of TrkPC1 peptide, but not of its truncated form, enhanced the ATP-evoked cytosolic Ca2+ concentrations. When Ca2+ assays were performed in HeLa cells characterized by Ca2+ stores greater than those of HEK-293 cells, the histamine-evoked cytosolic Ca2+ increase was enhanced by TrkPC1 expression, even in absence of external Ca2+. These observations indicate that the C-terminal tail of PC1 in kidney and other epithelial cells upregulates a Ca2+ channel activity also involved in the release of intracellular stores. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:657 / 664
页数:8
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