Rapid quantification of DNA libraries for next-generation sequencing

被引：22

作者：

Buehler, Bernd ^{[1
]}

Hogrefe, Holly H. ^{[1
]}

Scott, Graham ^{[2
]}

Ravi, Harini ^{[2
]}

Pabon-Pena, Carlos ^{[3
]}

O'Brien, Scott ^{[1
]}

Formosa, Rachel ^{[1
]}

Happe, Scott ^{[2
]}

机构：

[1] Agilent Technol, La Jolla, CA 92037 USA

[2] Agilent Technol, Cedar Creek, TX 78612 USA

[3] Agilent Technol, Santa Clara, CA 95051 USA

来源：

METHODS | 2010年 / 50卷 / 04期

关键词：

D O I：

10.1016/j.ymeth.2010.01.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The next-generation DNA sequencing workflows require an accurate quantification of the DNA molecules to be sequenced which assures optimal performance of the instrument. Here, we demonstrate the use of qPCR for quantification of DNA libraries used in next-generation sequencing. In addition, we find that qPCR quantification may allow improvements to current NGS workflows, including reducing the amount of library DNA required, increasing the accuracy in quantifying amplifiable DNA, and avoiding amplification bias by reducing or eliminating the need to amplify DNA before sequencing. (C) 2010 Published by Elsevier Inc.

引用

页码：S15 / S18

页数：4

共 7 条

[1] [Anonymous], 2015, AGILENT HIGH SENSITI
[2] [Anonymous], Paired-end sequencing
[3] Amplification efficiency of thermostable DNA polymerases
Arezi, B
Xing, WM
Sorge, JA
Hogrefe, HH
[J]. ANALYTICAL BIOCHEMISTRY, 2003, 321 (02) : 226 - 235
[4] Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing
Gnirke, Andreas
Melnikov, Alexandre
Maguire, Jared
Rogov, Peter
LeProust, Emily M.
Brockman, William
Fennell, Timothy
Giannoukos, Georgia
Fisher, Sheila
Russ, Carsten
Gabriel, Stacey
Jaffe, David B.
Lander, Eric S.
Nusbaum, Chad
[J]. NATURE BIOTECHNOLOGY, 2009, 27 (02) : 182 - 189
[5] Kozarewa I, 2009, NAT METHODS, V6, P291, DOI [10.1038/NMETH.1311, 10.1038/nmeth.1311]
[6] A large genome center's improvements to the Illumina sequencing system
Quail, Michael A.
Kozarewa, Iwanka
Smith, Frances
Scally, Aylwyn
Stephens, Philip J.
Durbin, Richard
Swerdlow, Harold
Turner, Daniel J.
[J]. NATURE METHODS, 2008, 5 (12) : 1005 - 1010
[7] SURESELECT USER MANU

← 1 →