RNaseI from Escherichia coli cannot substitute for S-RNase in rejection of Nicotiana plumbaginifolia pollen

被引:16
作者
Beecher, B [1 ]
Murfett, J [1 ]
McClure, BA [1 ]
机构
[1] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA
基金
美国国家科学基金会;
关键词
Nicotiana; pollination; self-incompatibility; S-RNase; unilateral incompatibility;
D O I
10.1023/A:1005904230755
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Unilateral incompatibility often occurs between self-incompatible (SI) species and their self-compatible (SC) relatives. For example, SI Nicotiana alata rejects pollen from SC N. plumbaginifolia, but the reciprocal pollination is compatible. This interspecific pollen rejection system closely resembles intraspecific S-allele-specific pollen rejection. However, the two systems differ in degree of specificity. In SI, rejection is S-allele-specific, meaning that only a single S-RNase causes rejection of pollen with a specific S genotype. Rejection of N. plumbaginifolia pollen is less specific, occurring in response to almost any S-RNase. Here, we have tested whether a non-S-RNase can cause rejection of N. plumbaginifolia pollen. The Escherichia coli ma gene encoding RNaseI was engineered for expression in transgenic (N. plumbaginifolia x SC N. alata) hybrids. Expression levels and pollination behavior of hybrids expressing E. coli RNaseI were compared to controls expressing S-A2-RNase from N. alata. Immunoblot analysis and RNase activity assays showed that RNaseI and S-A2-RNase were expressed at comparable levels. However, expression of S-A2-RNase caused rejection of N. plumbaginifolia pollen, whereas expression of RNaseI did not. Thus, in this system, RNase activity alone is not sufficient for rejection of N. plumbaginifolia pollen. The results suggest that S-RNases may be specially adapted to function in pollen rejection.
引用
收藏
页码:553 / 563
页数:11
相关论文
共 50 条
  • [1] SEQUENCE VARIABILITY OF 3 ALLELES OF THE SELF-INCOMPATIBILITY GENE OF NICOTIANA-ALATA
    ANDERSON, MA
    MCFADDEN, GI
    BERNATZKY, R
    ATKINSON, A
    ORPIN, T
    DEDMAN, H
    TREGEAR, G
    FERNLEY, R
    CLARKE, AE
    [J]. PLANT CELL, 1989, 1 (05) : 483 - 491
  • [2] CLONING OF CDNA FOR A STYLAR GLYCOPROTEIN ASSOCIATED WITH EXPRESSION OF SELF-INCOMPATIBILITY IN NICOTIANA-ALATA
    ANDERSON, MA
    CORNISH, EC
    MAU, SL
    WILLIAMS, EG
    HOGGART, R
    ATKINSON, A
    BONIG, I
    GREGO, B
    SIMPSON, R
    ROCHE, PJ
    HALEY, JD
    PENSCHOW, JD
    NIALL, HD
    TREGEAR, GW
    COGHLAN, JP
    CRAWFORD, RJ
    CLARKE, AE
    [J]. NATURE, 1986, 321 (6065) : 38 - 44
  • [3] BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION
    BEVAN, M
    [J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (22) : 8711 - 8721
  • [4] CDNA CLONING AND MOLECULAR ANALYSIS OF 2 SELF-INCOMPATIBILITY ALLELES FROM APPLE
    BROOTHAERTS, W
    JANSSENS, GA
    PROOST, P
    BROEKAERT, WF
    [J]. PLANT MOLECULAR BIOLOGY, 1995, 27 (03) : 499 - 511
  • [5] EXPRESSION OF UNILATERAL INCOMPATIBILITY IN POLLEN OF LYCOPERSICON-PENNELLII IS DETERMINED BY MAJOR LOCI ON CHROMOSOME-1, CHROMOSOME-6 AND CHROMOSOME-10
    CHETELAT, RT
    DEVERNA, JW
    [J]. THEORETICAL AND APPLIED GENETICS, 1991, 82 (06) : 704 - 712
  • [6] SEQUENCE VARIABILITY AND DEVELOPMENTAL EXPRESSION OF S-ALLELES IN SELF-INCOMPATIBLE AND PSEUDO-SELF-COMPATIBLE PETUNIA
    CLARK, KR
    OKULEY, JJ
    COLLINS, PD
    SIMS, TL
    [J]. PLANT CELL, 1990, 2 (08) : 815 - 826
  • [7] DEVELOPMENTALLY CONTROLLED EXPRESSION OF A GENE ASSOCIATED WITH SELF-INCOMPATIBILITY IN NICOTIANA-ALATA
    CORNISH, EC
    PETTITT, JM
    BONIG, I
    CLARKE, AE
    [J]. NATURE, 1987, 326 (6108) : 99 - 102
  • [8] DENETANCOURT D, 1977, INCOMPATIBILITY ANGI
  • [9] Molecular characterisation of an S-like RNase of Nicotiana alata that is induced by phosphate starvation
    Dodds, PN
    Clarke, AE
    Newbigin, E
    [J]. PLANT MOLECULAR BIOLOGY, 1996, 31 (02) : 227 - 238
  • [10] RELATEDNESS OF A PERIPLASMIC, BROAD-SPECIFICITY RNASE FROM AEROMONAS-HYDROPHILA TO RNASE-I OF ESCHERICHIA-COLI AND TO A FAMILY OF EUKARYOTIC RNASES
    FAVRE, D
    NGAI, PK
    TIMMIS, KN
    [J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (12) : 3710 - 3722