Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay

被引：46

作者：

Adachi, D

Johnson, G

Draker, R

Ayers, M

Mazzulli, T

Talbot, PJ

Tellier, R

机构：

[1] Hosp Sick Children, Div Microbiol, Toronto, ON M5G 1X8, Canada

[2] Hosp Sick Children, Metab Res Program, Toronto, ON M5G 1X8, Canada

[3] Mt Sinai Hosp, Dept Microbiol, Toronto, ON, Canada

[4] Inst Armand Frappier, INRS, Laval, PQ, Canada

来源：

JOURNAL OF VIROLOGICAL METHODS | 2004年 / 122卷 / 01期

基金：

加拿大健康研究院;

关键词：

coronavirus; human coronaviruses; RT-PCR assay; SARS;

D O I：

10.1016/j.jviromet.2004.07.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：29 / 36

页数：8

共 29 条

[1]

Drazen Jeffrey M, 2003, N Engl J Med, V348, pe6, DOI 10.1056/NEJMe030062

[2] Identification of a novel coronavirus in patients with severe acute respiratory syndrome [J].

Drosten, C ;

Günther, S ;

Preiser, W ;

van der Werf, S ;

Brodt, HR ;

Becker, S ;

Rabenau, H ;

Panning, M ;

Kolesnikova, L ;

Fouchier, RAM ;

Berger, A ;

Burguière, AM ;

Cinatl, J ;

Eickmann, M ;

Escriou, N ;

Grywna, K ;

Kramme, S ;

Manuguerra, JC ;

Müller, S ;

Rickerts, V ;

Stürmer, M ;

Vieth, S ;

Klenk, HD ;

Osterhaus, ADME ;

Schmitz, H ;

Doerr, HW .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1967-1976

[3] A previously undescribed coronavirus associated with respiratory disease in humans [J].

Fouchier, RAM ;

Hartwig, NG ;

Bestebroer, TM ;

Niemeyer, B ;

de Jong, JC ;

Simon, JH ;

Osterhaus, ADME .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (16) :6212-6216

[4] Aetiology -: Koch's postulates fulfilled for SARS virus [J].

Fouchier, RAM ;

Kuiken, T ;

Schutten, M ;

van Amerongen, G ;

van Doornum, J ;

van den Hoogen, BG ;

Peiris, M ;

Lim, W ;

Stöhr, K ;

Osterhaus, ADME .

NATURE, 2003, 423 (6937) :240-240

[5] Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome [J].

Guan, Y ;

Peiris, JSM ;

Zheng, B ;

Poon, LLM ;

Chan, KH ;

Zeng, FY ;

Chan, CWM ;

Chan, MN ;

Chen, JD ;

Chow, KYC ;

Hon, CC ;

Hui, KH ;

Li, J ;

Li, VYY ;

Wang, Y ;

Leung, SW ;

Yuen, KY ;

Leung, FC .

LANCET, 2004, 363 (9403) :99-104

[6]

HOLMES KV, 2001, FIELDS VIROLOGY, P1197

[7] Comprehensive PCR-based assay for detection and species identification of human herpesviruses [J].

Johnson, G ;

Nelson, S ;

Petric, M ;

Tellier, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (09) :3274-3279

[8] A novel coronavirus associated with severe acute respiratory syndrome [J].

Ksiazek, TG ;

Erdman, D ;

Goldsmith, CS ;

Zaki, SR ;

Peret, T ;

Emery, S ;

Tong, SX ;

Urbani, C ;

Comer, JA ;

Lim, W ;

Rollin, PE ;

Dowell, SF ;

Ling, AE ;

Humphrey, CD ;

Shieh, WJ ;

Guarner, J ;

Paddock, CD ;

Rota, P ;

Fields, B ;

DeRisi, J ;

Yang, JY ;

Cox, N ;

Hughes, JM ;

LeDuc, JW ;

Bellini, WJ ;

Anderson, LJ .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1953-1966

[9] Severe acute respiratory syndrome (SARS) in a liver transplant recipient and guidelines for donor SARS screening [J].

Kumar, D ;

Tellier, R ;

Draker, R ;

Levy, G ;

Humar, A .

AMERICAN JOURNAL OF TRANSPLANTATION, 2003, 3 (08) :977-981

[10] A major outbreak of severe acute respiratory syndrome in Hong Kong [J].

Lee, N ;

Hui, D ;

Wu, A ;

Chan, P ;

Cameron, P ;

Joynt, GM ;

Ahuja, A ;

Yung, MY ;

Leung, CB ;

To, KF ;

Lui, SF ;

Szeto, CC ;

Chung, S ;

Sung, JJY .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1986-1994

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