Neuroprotective effect of a ginseng (Panax ginseng) root extract on astrocytes primary culture

被引：57

作者：

Naval, M. V. ^{[1
]}

Gomez-Serranillos, M. P. ^{[1
]}

Carretero, M. E. ^{[1
]}

Villar, A. M. ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Farm, Dept Farmacol, E-28040 Madrid, Spain

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2007年 / 112卷 / 02期

关键词：

ginseng; ginsenosides; astrocytes; primary culture; hydrogen peroxide; antioxidants;

D O I：

10.1016/j.jep.2007.03.010

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A standardized aqueous extract of Panax ginseng radix was tested for its antioxidant effect on primary astrocytes culture on an oxidant stress model generated by H2O2. The results indicated that this extract had a significant effect on the reduction of astrocytic death induced by H2O2. Dose-response experiments revealed that this ginseng extract increased cell viability at a wide range of concentrations. Therefore, we investigated the effects of this extract on antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidases (GPx) and glutathione reductase (GR), on glutathione content (reduced and oxidized forms and red/ox index) and on the intracellular reactive oxygen species (ROS) formation. Exposure of astrocytes to H2O2 decreased the activities of antioxidant enzymes, and increased ROS formation. Ginseng root extract reversed the effect of almost all of these parameters in H2O2-injured primary cultures of rat astrocytes. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：262 / 270

页数：9

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