Large-scale preparation of the Δ10 form of staphylokinase by in vitro processing of recombinant staphylokinase with purified human plasminogen

被引：3

作者：

Chattopadhyay, D ^{[1
]}

Stewart, JE ^{[1
]}

DeLucas, LJ ^{[1
]}

机构：

[1] Univ Alabama, Ctr Macromol Crystallog, Birmingham, AL 35294 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 69卷 / 03期

基金：

美国国家航空航天局;

关键词：

plasminogen; plasminogen activator; staphylokinase; immobilized metal affinity chromatography; hydrophobic interaction chromatography; recombinant protein;

D O I：

10.1007/BF02788810

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The authors have developed a rapid and convenient method for purification of a low molecular weight form (Delta 10) of the bacterial plasminogen activator, staphylokinase. Recombinant staphylokinase is expressed in Escherichia coli, with an amino terminal extension that facilitated purification by immobilized metal-affinity chromatography. Purified staphylokinase is treated with human plasminogen, and the resulting truncated form is purified using a combination of immobilized metal affinity chromatography and hydrophobic interaction chromatography. Purified protein is characterized by amino terminal sequencing and in vitro plasminogen activation assay.

引用

页码：147 / 156

页数：10

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