Expression of long-patch and short-patch DNA mismatch repair proteins in the embryonic and adult mammalian brain

被引:32
作者
Marietta, C
Palombo, F
Gallinari, P
Jiricny, J
Brooks, PJ
机构
[1] NIAAA, Mol Neurobiol Sect, Neurogenet Lab, NIH,DICBR,LING, Bethesda, MD 20892 USA
[2] Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, Italy
[3] Inst Med Radiobiol, CH-8029 Zurich, Switzerland
来源
MOLECULAR BRAIN RESEARCH | 1998年 / 53卷 / 1-2期
基金
美国国家卫生研究院;
关键词
cerebellum; DNA repair; cell nucleus; deamination; DNA replication; brain development;
D O I
10.1016/S0169-328X(97)00311-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Expression of the DNA mismatch repair (MMR) pathway was examined in the adult and developing rat brain. Rat homologues of human GTBP and MSH2, which are essential components of the post-replicative DNA MMR system, were identified in nuclear extracts from the adult and developing rat brain. Developmental studies showed that both GTBP and MSH2 levels were higher in nuclei isolated from the embryonic brain (day 16) than adult brain. However, this difference was not as dramatic as the difference in the number of proliferating cells. Levels of thymine DNA glycosylase (TDG), the enzyme which catalyzes the first step in short patch G:T mismatch repair, were also decreased in adult compared to embryonic brain. In the adult brain, MMR proteins were elevated in nuclear extracts enriched for neuronal nuclei. These results suggest that adult brain cells have the capacity to carry out DNA mismatch repair, in spite of a lack of ongoing DNA replication. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:317 / 320
页数:4
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