Impact of glycosylation on the immunogenicity of a DNA-based influenza H5HA vaccine

被引:65
作者
Bright, RA
Ross, TM
Subbarao, K
Robinson, HL
Katz, JM
机构
[1] Ctr Dis Control & Prevent, Influenza Branch, Div Viral & Rickettsial Dis, Atlanta, GA 30333 USA
[2] Emory Univ, Emory Vaccine Res Ctr, Yerkes Primate Res Ctr, Atlanta, GA 30329 USA
[3] E Carolina Univ, Sch Med, Dept Microbiol & Immunol, Greenville, NC 27858 USA
关键词
H5N1; avian influenza; DNA vaccines; hemagglutinin; glycosylation;
D O I
10.1016/S0042-6822(03)00008-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Avian H5N1 influenza viruses isolated from humans in Hong Kong in 1997 were divided into two antigenic groups based on the presence or absence of a potential glycosylation site at amino acid residues 154-156 in the HA1 region of the viral hemagglutinin (HA) surface glycoprotein. To assess the impact of glycosylation on the immunogenicity of an HA-expressing DNA vaccine, a series of plasmid vaccine constructs that differed in the presence of potential glycosylation sites at amino acid residues 154-156, 165-167, and 286-288 were used to immunize BALB/c mice. Postvaccination serum I-G, hemagglutination inhibition, and neutralizing antibody titers as well as the morbidity and mortality following a lethal H5N1 viral challenge did not vary significantly among any of the experimental groups. We conclude that the glycosylation pattern of the influenza virus HA1 domain has little impact on the murine antibody response raised to a DNA vaccine encoding the H5 HA, thereby minimizing the concern that the pattern of glycosylation sites encoded by the vaccine match those of closely related H5 viruses. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:270 / 278
页数:9
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