Electron tomography of single ice-embedded macromolecules: Three-dimensional alignment and classification

被引：79

作者：

Walz, J ^{[1
]}

Typke, D ^{[1
]}

Nitsch, M ^{[1
]}

Koster, AJ ^{[1
]}

Hegerl, R ^{[1
]}

Baumeister, W ^{[1
]}

机构：

[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1997年 / 120卷 / 03期

关键词：

D O I：

10.1006/jsbi.1997.3934

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level. (C) 1997 Academic Press.

引用

页码：387 / 395

页数：9

共 24 条

[1] Determination of the fold of the core protein of hepatitis B virus ky electron cryomicroscopy [J].