Abiotic and enzymatic degradation of wheat straw cell wall: a biochemical and ultrastructural investigation

被引:14
作者
Lequart, C
Ruel, K
Lapierre, C
Pollet, B
Kurek, B
机构
[1] INRA, UPBP, Equipe Biochim Macromol Vegetales, F-51686 Reims 2, France
[2] INRA, UPBP, Equipe Fractionnement Enzymat, F-51686 Reims 2, France
[3] CERMAV, Lab Biochim Parois Vegetales, CNRS, Grenoble, France
[4] INRA, Biochim Lab, INA PG, Grignon, France
[5] INRA, UPBP, Equipe Biochim Macromol Vegetales, F-51686 Reims 2, France
关键词
biodegradation; lignins; hemicelluloses; Mn chelate; xylanase; transmission electron microscopy;
D O I
10.1016/S0168-1656(00)00267-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The action of an abiotic lignin oxidant and a diffusible xylanase on wheat straw was studied and characterized at the levels of the molecular structures by chemical analysis and of the cell wall ultrastructure by transmission electron microscopy. While distinct chemical changes in the target polymers were observed when each system was used separately, a combination of the two types of catalysts did not significantly increase either lignin oxidation or hemicellulose hydrolysis. Microscopic observations however revealed that the supramolecular organization of the cell wall polymers was significantly altered. This suggests that the abiotic Mn-oxalate complex and the xylanase cooperate in modifying the cell wall architecture, without noticeably enhancing the degradation of the constitutive polymers. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:249 / 259
页数:11
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