Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by native microflora and combinations of white-rot fungi in a coal-tar contaminated soil

被引:117
作者
Canet, R
Birnstingl, JG
Malcolm, DG
Lopez-Real, JM
Beck, AJ
机构
[1] Imperial Coll Wye, Dept Biol, Ashford TN25 5AH, Kent, England
[2] Dames & Moore, London SW19 4DR, England
[3] British Aerosp Def Ltd, Royal Ordnance Div, Chorley PR7 6AD, Lancs, England
关键词
white-rot fungi; polycyclic aromatic hydrocarbons (PAHs); coal tar; biodegradation; microcosms;
D O I
10.1016/S0960-8524(00)00093-6
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Four white-rot fungi (Phanerochaete chrysosporium IMI 232175, Pleurotus ostreatus from the University of Alberta Microfungus Collection IMI 341687, Coriolus versicolor IMI 210866 and Wye isolate #7) and all possible combinations of two or more of these fungi, were incubated in microcosms containing wheat straw and non-sterile coal-tar contaminated soil to determine their potential to degrade polycyclic aromatic hydrocarbons (PAHs). Biotic and abiotic controls were prepared similarly and PAH concentrations remaining in each microcosm were determined after 8, 16 and 32 weeks by GC-MS following extraction with dichloromethane. The greatest PAH losses were in the biotic control, compared to small or negligible differences in microcosms inoculated with one or more fungi. These results suggest that in the biotic control native microorganisms colonised the straw added as organic substrate and degraded PAH as an indirect consequence of their metabolism. By contrast, in other microcosms, colonisation of straw by the natural microflora was inhibited because the straw was previously inoculated with fungi. Soil cultures prepared at the end of the experiment showed that though introduced fungi were still alive, they were unable to thrive and degrade PAH in such a highly contaminated soil and remained in a metabolically inactive form. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
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页码:113 / 117
页数:5
相关论文
共 17 条
[2]  
BIRNSTINGL JGA, 1997, THESIS U LANCASTER U
[3]   ENHANCED BIODEGRADATION OF PHENANTHRENE IN OIL TAR-CONTAMINATED SOILS SUPPLEMENTED WITH PHANEROCHAETE-CHRYSOSPORIUM [J].
BRODKORB, TS ;
LEGGE, RL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (09) :3117-3121
[4]  
Canet R., 1999, Land Contamination & Reclamation, V7, P191
[5]   FIELD-EVALUATION OF THE LIGNIN-DEGRADING FUNGUS PHANEROCHAETE-SORDIDA TO TREAT CREOSOTE-CONTAMINATED SOIL [J].
DAVIS, MW ;
GLASER, JA ;
EVANS, JW ;
LAMAR, RT .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1993, 27 (12) :2572-2576
[6]   BIODEGRADATION OF POLYCYCLIC AROMATIC-HYDROCARBONS BY NEW ISOLATES OF WHITE ROT FUNGI [J].
FIELD, JA ;
DEJONG, E ;
COSTA, GF ;
DEBONT, JAM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (07) :2219-2226
[7]  
GOLD MH, 1988, METHOD ENZYMOL, V161, P74
[8]   Two-step degradation of pyrene by white-rot fungi and soil microorganisms [J].
inderWiesche, C ;
Martens, R ;
Zadrazil, F .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 46 (5-6) :653-659
[9]   Ex-situ process for treating PAH-contaminated soil with Phanerochaete chrysosporium [J].
May, R ;
Schroder, P ;
Sandermann, H .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1997, 31 (09) :2626-2633
[10]   REMEDIATION OF PETROLEUM IMPACTED SOILS IN FUNGAL COMPOST BIOREACTORS [J].
MCFARLAND, MJ ;
QIU, XJ ;
SIMS, JL ;
RANDOLPH, ME ;
SIMS, RC .
WATER SCIENCE AND TECHNOLOGY, 1992, 25 (03) :197-206