Role for caspases in lens fiber differentiation

被引:242
作者
Ishizaki, Y
Jacobson, MD
Raff, MC [1 ]
机构
[1] UCL, MRC, Mol Cell Biol Lab, London WC1E 6BT, England
[2] UCL, Dept Biol, London WC1E 6BT, England
[3] Kobe Univ, Sch Med, Dept Hyg, Chuo Ku, Kobe, Hyogo 650, Japan
关键词
D O I
10.1083/jcb.140.1.153
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
There is increasing evidence that programmed cell death (PCD) depends on a novel family of intracellular cysteine proteases, called caspases, that includes the Ced-3 protease in the nematode Caenorhabditis elegans and the interleukin-1 beta-converting enzyme (ICE)-like proteases in mammals, Some developing cells, including lens epithelial cells, erythroblasts, and keratinocytes, lose their nucleus and other organelles when they terminally differentiate, but it is not known whether the enzymatic machinery of PCD is involved in any of these normal differentiation events. We show here that at least one CPP32 (caspase-3)-like member of the caspase family becomes activated when rodent lens epithelial cells terminally differentiate into anucleate lens fibers in vivo, and that a peptide inhibitor of these proteases blocks the denucleation process in an in vitro model of lens fiber differentiation. These findings suggest that at least part of the machinery of PCD is involved in lens fiber differentiation.
引用
收藏
页码:153 / 158
页数:6
相关论文
共 48 条
[1]   Human ICE/CED-3 protease nomenclature [J].
Alnemri, ES ;
Livingston, DJ ;
Nicholson, DW ;
Salvesen, G ;
Thornberry, NA ;
Wong, WW ;
Yuan, JY .
CELL, 1996, 87 (02) :171-171
[2]   DNA-DEGRADATION IN TERMINALLY DIFFERENTIATING LENS FIBER CELLS FROM CHICK-EMBRYOS [J].
APPLEBY, DW ;
MODAK, SP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5579-5583
[3]   Chromatin degradation in differentiating fiber cells of the eye lens [J].
Bassnett, S ;
Mataic, D .
JOURNAL OF CELL BIOLOGY, 1997, 137 (01) :37-49
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]  
Chinnaiyan AM, 1996, J BIOL CHEM, V271, P4573
[6]  
Chow RL, 1995, DEVELOPMENT, V121, P4383
[7]   INVOLVEMENT OF MULTIPLE PROTEASES DURING FAS-MEDIATED APOPTOSIS IN T-LYMPHOCYTES [J].
CHOW, SC ;
WEIS, M ;
KASS, GEN ;
HOLMSTROM, TH ;
ERIKSSON, JE ;
ORRENIUS, S .
FEBS LETTERS, 1995, 364 (02) :134-138
[8]   ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B [J].
Duan, HJ ;
Orth, K ;
Chinnaiyan, AM ;
Poirier, GG ;
Froelich, CJ ;
He, WW ;
Dixit, VM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (28) :16720-16724
[9]   MECHANISMS AND FUNCTIONS OF CELL-DEATH [J].
ELLIS, RE ;
YUAN, JY ;
HORVITZ, HR .
ANNUAL REVIEW OF CELL BIOLOGY, 1991, 7 :663-698
[10]  
FERNANDESALNEMRI T, 1995, CANCER RES, V55, P6045