Identification and nucleotide sequence analysis of a cryptic plasmid, pRM21, from Rhodothermus marinus

被引：5

作者：

Ernstsson, S ^{[1
]}

Bjornsdottir, SH ^{[1
]}

Jónsson, ZO ^{[1
]}

Thorbjarnardottir, SH ^{[1
]}

Eggertsson, G ^{[1
]}

Palsdottir, A ^{[1
]}

机构：

[1] Univ Iceland, Inst Biol, Genet Mol Lab, IS-108 Reykjavik, Iceland

来源：

PLASMID | 2003年 / 49卷 / 02期

关键词：

plasmid; Rhodothermus marinus; nucleotide sequence; thermophile;

D O I：

10.1016/S0147-619X(02)00152-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Here we report the identification and nucleotide sequence analysis of pRM21, a plasmid isolated from the thermophilic eubacterium Rhodothermus marinus. pRM21 consists of 2935 bp, has a G+C content of 58.2% and one major open reading frame whose deduced product shows significant similarities to RepA proteins from several plasmids, the highest being to the RepA of pSa from Escherichia coli. A region with the characteristics of iteron-containing replicons, three 19 bp repeats, DnaA boxes, an A+T rich region and GATC sequences, was identified. Of 40 additional R marinus strains screened for plasmids, six (15%) were found to harbour plasmids with the same size and restriction pattern as pRM21. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：188 / 191

页数：4

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