Malignant transformation of B lymphoma cell line BJAB by Epstein-Barr virus-encoded small RNAs

被引：65

作者：

Yamamoto, N

Takizawa, T

Iwanaga, Y

Shimizu, N

Yamamoto, N

机构：

[1] Tokyo Med & Dent Univ, Sch Med, Dept Microbiol & Mol Virol, Bunkyo Ku, Tokyo 1138519, Japan

[2] Aichi Human Serv Ctr, Inst Dev Res, Dept Biochem, Kasugai, Aichi 4800392, Japan

[3] Tokyo Med & Dent Univ, Med Res Inst, Dept Virol, Bunkyo Ku, Tokyo 1138519, Japan

来源：

FEBS LETTERS | 2000年 / 484卷 / 02期

关键词：

Epstein-Barr virus; Epstein-Barr virus-encoded small RNA; double-stranded RNA-dependent protein; kinase; apoptosis; transformation;

D O I：

10.1016/S0014-5793(00)02145-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

EBV-encoded small RNAs (EBERs) are non-polyadenylated and abundantly transcribed RNAs, whose functions have not yet been fully elucidated. Here, we report that the EBV-negative B lymphoma cell line, BJAB, was rendered more malignant and resistant to apoptosis by EBERs, EBER-transfected cells exhibited enhanced growth potential in SCID mice as well as in soft agar, and showed resistance to apoptotic stimuli in comparison with the vector control. EBERs inhibited the activity of the double-stranded RNA-dependent protein kinase, PKR, which is reputed to art as a tumor-suppressor. These results suggest that EBERs play an important role in the pathogenesis of EBV-associated malignancies through the inhibition of PKR, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：153 / 158

页数：6

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