ZAP-70 intron1 DNA methylation status: Determination by pyrosequencing in B chronic lymphocytic leukemia

被引：19

作者：

Chantepie, Sylvain P. ^{[1
,2
,3
]}

Vaur, Dominique ^{[2
,3
]}

Grunau, Christoph ^{[4
]}

Salauen, Veronique ^{[5
]}

Briand, Melanie ^{[2
]}

Parienti, Jean-Jacques ^{[6
]}

Heutte, Natacha ^{[2
]}

Cheze, Stephane

Roussel, Mikel ^{[7
]}

Gauduchon, Pascal ^{[2
]}

Leporrier, Michel

Krieger, Sophie ^{[2
,3
]}

机构：

[1] Univ Caen Basse Normandie, Hematol Serv, CHU Caen, Hematol Clin, F-14033 Caen, France

[2] Univ Caen Basse Normandie, Ctr Francois Baclesse, GRECAN, F-14033 Caen, France

[3] Univ Caen Basse Normandie, Ctr Francois Baclesse, Lab Biol Clin & Oncol, F-14033 Caen, France

[4] CNRS, UMR 5244, Pergpignan, France

[5] CHU Caen, Hematol Lab, F-14000 Caen, France

[6] CHU Caen, Serv Rech Clin & Biostat, F-14000 Caen, France

[7] CHU Pontchaillou Rennes, Hematol Lab, Rennes, France

来源：

LEUKEMIA RESEARCH | 2010年 / 34卷 / 06期

关键词：

ZAP-70; CLL; DNA methylation; Pyrosequencing; Epigenetics; VARIABLE-REGION MUTATIONS; EXPRESSION; DISEASE; CD38; PHENOTYPE; SURVIVAL; REVEALS; CANCER; GENES; CELLS;

D O I：

10.1016/j.leukres.2009.10.018

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

ZAP-70 expression is a strong prognostic indicator in chronic lymphocytic leukemia. However, ZAP-70 quanti. cation by flow cytometry lacks sufficient standardization. Based upon the correlation between ZAP-70 expression and its gene methylation status, we have developed a quantitative pyrosequencing assay for the determination of ZAP-70 methylation adapted for routine use. Methylation in four CpG pairs (C-223, C-243, C-254, and C-267) in the first intron of ZAP-70 is associated with repression of ZAP-70. Moreover, it correlates with CD38 expression (n = 111, p<.0001), IgHv mutation status (n = 106, p<.0001), time to treatment (p<.0001), and overall survival (p=.0014). Pyrosequencing of ZAP-70 provides a good alternative to flow cytometry. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：800 / 808

页数：9

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