A spin cartridge system for DNA extraction from paraffin wax embedded tissues

被引:12
作者
Pinto, AP
Villa, LL
机构
[1] Ludwig Inst Canc Res, Dept Virol, BR-01509010 Sao Paulo, Brazil
[2] Univ Fed Parana, Dept Pathol, BR-80060000 Curitiba, Parana, Brazil
来源
JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY | 1998年 / 51卷 / 01期
关键词
paraffin wax embedded tissues; DNA extraction; polymerase chain reaction; human papillomavirus;
D O I
10.1136/mp.51.1.48
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
A simple and efficient method of DNA extraction from paraffin wax embedded tissues using a spin cartridge system is described. Such DNAs were shown to be suitable for amplification by the polymerase chain reaction, which targeted two human papillomavirus genes and one globin fragment giving rise to products of 450, 150, and 110 base pairs, respectively. Different human tissues, stored for up to 20 years, were successfully amplified, demonstrating the usefulness of this very simple procedure for retrospective studies.
引用
收藏
页码:48 / 49
页数:2
相关论文
共 10 条
  • [1] BANERJEE SK, 1995, BIOTECHNIQUES, V18, P768
  • [2] RAPID PREPARATION OF TISSUE DNA FROM PARAFFIN-EMBEDDED BLOCKS AND ANALYSIS BY POLYMERASE CHAIN-REACTION
    CHEN, BF
    CLEJAN, S
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1993, 41 (05) : 765 - 768
  • [3] HUSMAN AMDR, 1995, J GEN VIROL, V76, P1057
  • [4] TISSUE EXTRACTION OF DNA AND RNA AND ANALYSIS BY THE POLYMERASE CHAIN-REACTION
    JACKSON, DP
    LEWIS, FA
    TAYLOR, GR
    BOYLSTON, AW
    QUIRKE, P
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 1990, 43 (06) : 499 - 504
  • [5] KALAVATHY S, 1996, FOCUS, V18, P22
  • [6] McGee JOD., 1992, DIAGNOSTIC MOL PATHO, P131
  • [7] Morgan K, 1996, J CLIN PATHOL-CL MOL, V49, pM179
  • [8] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE
    SAIKI, RK
    GELFAND, DH
    STOFFEL, S
    SCHARF, SJ
    HIGUCHI, R
    HORN, GT
    MULLIS, KB
    ERLICH, HA
    [J]. SCIENCE, 1988, 239 (4839) : 487 - 491
  • [9] Sanguinetti CJ, 1994, BIOTECHNIQUES, V17, P915
  • [10] Wright DK., 1990, PCR PROTOCOLS GUIDE, V19, P153