Expression of c-met proto-oncogene in primary colorectal cancer and liver metastases

被引:61
作者
Fujita, S [1 ]
Sugano, K [1 ]
机构
[1] Natl Canc Ctr Hosp, Dept Surg, Chuo Ku, Tokyo 104, Japan
关键词
c-met; colorectal cancer; liver metastasis; hepatocyte growth factor; reverse transcriptase polymerase chain reaction;
D O I
10.1093/jjco/27.6.378
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have examined the expression of c-met mRNA in tissue from 27 colorectal cancers and ten liver metastases using the reverse transcriptase-polymerase chain reaction method. The expression of c-met mRNA in these tissues was quantified and the copy number of c-met mRNA to 10(8.0) copies of beta-actin mRNA was calculated, Mean copy numbers of c-met mRNA in cancer tissue and normal mucosa were 10(5.5) and 10(4.5) respectively, The c-met expression of cancer was significantly higher than that of normal mucosa (P < 0.0001), In 20 of 22 samples in which c-met expression of both tumor and corresponding normal tissue were examined, c-met was overexpressed in the cancer tissue, No correlation was found between c-met expression and the clinicopathologic background, The mean copy numbers of c-met mRNA in the tissue from the ten liver metastases and normal liver were 10(6.1) and 10(6.2) respectively. Although c-met expression in metastatic tissue was higher than that in the primary cancer tissue, the increase was not statistically significant. In three of four patients with synchronous liver metastases, c-met was overexpressed in the metastatic tissue compared with that in the corresponding primary cancer tissue, These results show that c-met is overexpressed in both primary colorectal cancer and liver metastases and suggest that c-met plays a role in the development of colorectal cancer liver metastases.
引用
收藏
页码:378 / 383
页数:6
相关论文
共 31 条
[1]  
BOCCACCIO C, 1994, J BIOL CHEM, V269, P12846
[2]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[3]   THE HUMAN MET ONCOGENE IS RELATED TO THE TYROSINE KINASE ONCOGENES [J].
DEAN, M ;
PARK, M ;
LEBEAU, MM ;
ROBINS, TS ;
DIAZ, MO ;
ROWLEY, JD ;
BLAIR, DG ;
VANDEWOUDE, GF .
NATURE, 1985, 318 (6044) :385-388
[4]  
DIRENZO MF, 1995, CANCER RES, V55, P1129
[5]  
DIRENZO MF, 1995, CLIN CANCER RES, V1, P147
[6]  
DIRENZO MF, 1992, ONCOGENE, V7, P2549
[7]  
EBERT M, 1994, CANCER RES, V54, P5775
[8]  
GHERARDI E, 1991, CANCER CELL-MON REV, V3, P227
[9]   P53 AND BEHAVIOR OF COLORECTAL-CANCER [J].
GOH, HS ;
CHAN, CS ;
KHINE, K ;
SMITH, DR .
LANCET, 1994, 344 (8917) :233-234
[10]   PURIFICATION AND PARTIAL CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR FROM PLASMA OF A PATIENT WITH FULMINANT HEPATIC-FAILURE [J].
GOHDA, E ;
TSUBOUCHI, H ;
NAKAYAMA, H ;
HIRONO, S ;
SAKIYAMA, O ;
TAKAHASHI, K ;
MIYAZAKI, H ;
HASHIMOTO, S ;
DAIKUHARA, Y .
JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (02) :414-419