High-resolution imaging of single fluorescent molecules with the optical near-field of a metal tip

被引:184
作者
Frey, HG [1 ]
Witt, S [1 ]
Felderer, K [1 ]
Guckenberger, R [1 ]
机构
[1] Max Planck Inst Biochem, Abt Mol Strukturbiol, D-82152 Martinsried, Germany
关键词
D O I
10.1103/PhysRevLett.93.200801
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
We show that a concentration of light at a metal tip allows near-field optical imaging of single fluorescent dye molecules at very high resolution, despite strong quenching effects. Details as small as 10 nm were observed in the fluorescence patterns of single Cy-3 dyes bound to the termini of DNA. Data evaluation by model fitting determines the positions of the dyes to an accuracy even better than 1 nm and also yields their 3D orientation. The metal tip simultaneously provides high-resolution topographic imaging complementing the optical signal for a detailed surface examination.
引用
收藏
页码:200801 / 1
页数:4
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