Interaction between hydrogen peroxide and plant hormones during germination and the early growth of pea seedlings

被引：181

作者：

Barba-Espin, G. ^{[1
]}

Diaz-Vivancos, P. ^{[1
]}

Clemente-Moreno, M. J. ^{[1
]}

Albacete, A. ^{[2
]}

Faize, L. ^{[1
]}

Faize, M. ^{[3
]}

Perez-Alfocea, F. ^{[2
]}

Hernandez, J. A. ^{[1
]}

机构：

[1] CSIC, Ctr Edafol & Biol Aplicada Segura, Grp Biotecnol Frutales, Dept Mejora Vegetal, E-30100 Murcia, Spain

[2] CEBAS CSIC, Dept Nutr Vegetal, Murcia 30100, Spain

[3] Univ Chouaib Doukkali, Lab Biol &Biotechnol Vegetales, Fac Sci, El Jadida 24000, Morocco

来源：

PLANT CELL AND ENVIRONMENT | 2010年 / 33卷 / 06期

关键词：

Pisum sativum; 2-DE; abcisic acid; antioxidative metabolism; ascorbate peroxidase; cytokinins; CONTROLLED TUMOR PROTEIN; ARABIDOPSIS SEED-GERMINATION; SALICYLIC-ACID; OXIDATIVE STRESS; ABSCISIC-ACID; ASCORBATE METABOLISM; ANTIOXIDANT ENZYMES; CELL ELONGATION; GENE-EXPRESSION; IN-VIVO;

D O I：

10.1111/j.1365-3040.2010.02120.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Hydrogen peroxide (H2O2) increased the germination percentage of pea seeds, as well as the growth of seedlings in a concentration-dependent manner. The effect of H2O2 on seedling growth was removed by incubation with 10 mu m ABA. The H2O2-pretreatment produced an increase in ascorbate peroxidase (APX), peroxidase (POX) and ascorbate oxidase (AAO). The increases in these ascorbate-oxidizing enzymes correlated with the increase in the growth of the pea seedlings as well as with the decrease in the redox state of ascorbate. Moreover, the increase in APX activity was due to increases in the transcript levels of cytosolic and stromal APX (cytAPX, stAPX). The proteomic analysis showed that H2O2 induced proteins related to plant signalling and development, cell elongation and division, and cell cycle control. A strong correlation between the effect of H2O2 on plant growth and the decreases in ABA and zeatin riboside (ZR) was observed. The results suggest an interaction among the redox state and plant hormones, orchestrated by H2O2, in the induction of proteins related to plant signalling and development during the early growth of pea seedlings.

引用

页码：981 / 994

页数：14

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