Sequence analysis and biochemical characterisation of chromosomal CAV-1 (Aeromonas caviae), the parental cephalosporinase of plasmid-mediated AmpC 'FOX' cluster

被引：31

作者：

Fosse, T

Giraud-Morin, C

Madinier, I

Labia, R

机构：

[1] CHU Nice, Hop Archet 2, Bacteriol Lab, F-06202 Nice 3, France

[2] CNRS, UMR 175, F-29000 Quimper, France

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 222卷 / 01期

关键词：

beta-lactamase; cephalosporin; beta-lactam resistance; Aeromonas;

D O I：

10.1016/S0378-1097(03)00253-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aeromonas cavide CIP 74.32 was resistant to amoxicillin, ticarcillin and cephalothin, and susceptible to cefoxitin, cefotaxime, ceftazidime, aztreonam and imipenem. This strain produced a cephalosporinase (pI 7.2) and an oxacillinase (pI 8.5). The cephalosporinase gene cav-1 was cloned and sequenced. Unlike A. caviae donor, Escherichia coli pNCE50 transformant producing CAV-1 beta-lactamase was resistant to cefoxitin. The deduced protein sequence CAV-1 contained 382 amino acids, and shared >96% homology with FOX-1 to FOX-5 cephalosporinase. CAV-1 presented only two amino acid substitutions (Thr270Ser and Arg271Ala) with FOX-1. CAV-1 is the chromosomal putative ancestor of the FOX family, a cluster of class C/group 1 plasmidic cephalosporinases spreading in Klebsiella and E. coli clinical isolates via conjugative plasmids. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：93 / 98

页数：6

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