MALDI-TOF MS of phosphatidylethanolamines:: Different adducts cause different post source decay (PSD) fragment ion spectra

被引：65

作者：

Fuchs, Beate ^{[1
]}

Schober, Celestina ^{[1
]}

Richter, Grit ^{[1
]}

Suess, Rosmarie ^{[1
]}

Schiller, Juergen ^{[1
]}

机构：

[1] Univ Leipzig, Fac Med, Inst Med Phys & Biophys, D-04107 Leipzig, Germany

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2007年 / 70卷 / 04期

关键词：

MALDI-TOF MS; phosphatidylethanolamine; post source decay; acyl chain composition;

D O I：

10.1016/j.jbbm.2007.03.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly applied to lipids. However, positional acyl chain analysis of lipids by MALDI was so far scarcely described. In this paper, the fragmentation behavior of phosphatidylethanolamine (PE) is investigated by using post-source decay (PSD) MS. In dependence on the investigated adduct, significant differences could be obtained. It will be shown that in particular the negative ion spectra enable the determination of the individual acyl chains as well as their positions (sn-1 or sn-2). Therefore, MALDI-TOF PSD spectra are a real alternative to more sophisticated MS/MS methods. (c) 2007 Elsevier B.V All rights reserved.

引用

页码：689 / 692

页数：4

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