Comparative mapping in the pig: localization of 214 expressed sequence tags

被引：14

作者：

Cirera, S

Jorgensen, CB

Sawera, M

Raudsepp, T

Chowdhary, BP

Fredholm, M

机构：

[1] Royal Vet & Agr Univ, Dept Anim Sci & Anim Hlth, Div Genet, DK-1870 Frederiksberg C, Denmark

[2] Texas A&M Univ, Dept Vet Anat & Publ Hlth, College Stn, TX 77843 USA

来源：

MAMMALIAN GENOME | 2003年 / 14卷 / 06期

关键词：

D O I：

10.1007/s00335-002-2242-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In total, 214 ESTs (Expressed Sequence Tags) were assigned to the porcine gene map by using somatic cell hybrid mapping, radiation hybrid mapping, and FISH. The ESTs were isolated from a porcine small intestine cDNA library on the basis of significant sequence identity with human annotated genes. in total, 390 primer pairs were designed primarily in the 3' UTR of the sequences. Overall, 58.6% of the ESTs were successfully mapped by this approach. In total, 191 of the localizations are in agreement with the human comparative map, strongly indicating that these represent true orthologous genes. The remaining 23 ESTs provide new comparative mapping data, which should be considered as preliminary until confirmed by other studies. Our mapping efforts provide a significant contribution to the porcine map as well as to the comparative map for human and pig.

引用

页码：405 / 426

页数：22

共 31 条

[1] A large-fragment porcine genomic library resource in a BAC vector [J].

Anderson, SI ;

Lopez-Corrales, NL ;

Gorick, B ;

Archibald, AL .

MAMMALIAN GENOME, 2000, 11 (09) :811-814

[2] An ordered comparative map of the cattle and human genomes [J].

Band, MR ;

Larson, JH ;

Rebeiz, M ;

Green, CA ;

Heyen, DW ;

Donovan, J ;

Windish, R ;

Steining, C ;

Mahyuddin, P ;

Womack, JE ;

Lewin, HA .

GENOME RESEARCH, 2000, 10 (09) :1359-1368

[3] Chromosome-specific single-locus FISH probes allow anchorage of an 1800-marker integrated radiation-hybrid/linkage map of the domestic dog genome to all chromosomes [J].

Breen, M ;

Jouquand, S ;

Renier, C ;

Mellersh, CS ;

Hitte, C ;

Holmes, NG ;

Chéron, A ;

Suter, N ;

Vignaux, F ;

Bristow, AE ;

Priat, C ;

McCann, E ;

André, C ;

Boundy, S ;

Gitsham, P ;

Thomas, R ;

Bridge, WL ;

Spriggs, HF ;

Ryder, EJ ;

Curson, A ;

Sampson, J ;

Ostrander, EA ;

Binns, MM ;

Galibert, F .

GENOME RESEARCH, 2001, 11 (10) :1784-1795

[4]

Chevalet C, 1997, COMPUT APPL BIOSCI, V13, P69

[5] FISH ON METAPHASE AND INTERPHASE CHROMOSOMES DEMONSTRATES THE PHYSICAL ORDER OF THE GENES FOR GPI, CRC, AND LIPE IN PIGS [J].

CHOWDHARY, BP ;

DELASENA, C ;

HARBITZ, I ;

ERIKSSON, L ;

GUSTAVSSON, I .

CYTOGENETICS AND CELL GENETICS, 1995, 71 (02) :175-178

[6] Construction of a 5000rad whole-genome radiation hybrid panel in the horse and generation of a comprehensive and comparative map for ECA11 [J].

Chowdhary, BP ;

Raudsepp, T ;

Honeycutt, D ;

Owens, EK ;

Piumi, F ;

Guérin, G ;

Matise, TC ;

Kata, SR ;

Womack, JE ;

Skow, LC .

MAMMALIAN GENOME, 2002, 13 (02) :89-94

[7] Expansion of the pig comparative map by expressed sequence tags (EST) mapping [J].

Fridolfsson, AK ;

Hori, T ;

Wintero, AK ;

Fredholm, M ;

Yerle, M ;

Robic, A ;

Andersson, L ;

Ellegren, H .

MAMMALIAN GENOME, 1997, 8 (12) :907-912

[8] STANDARD KARYOTYPE OF THE DOMESTIC PIG [J].

GUSTAVSSON, I .

HEREDITAS, 1988, 109 (02) :151-157

[9] A first-generation porcine whole-genome radiation hybrid map [J].

Hawken, RJ ;

Murtaugh, J ;

Flickinger, GH ;

Yerle, M ;

Robic, A ;

Milan, D ;

Gellin, J ;

Beattie, CW ;

Schook, LB ;

Alexander, LJ .

MAMMALIAN GENOME, 1999, 10 (08) :824-830

[10] A radiation hybrid map of mouse genes [J].

Hudson, TJ ;

Church, DM ;

Greenaway, S ;

Nguyen, H ;

Cook, A ;

Steen, RG ;

Van Etten, WJ ;

Castle, AB ;

Strivens, MA ;

Trickett, P ;

Heuston, C ;

Davison, C ;

Southwell, A ;

Hardisty, R ;

Varela-Carver, A ;

Haynes, AR ;

Rodriguez-Tome, P ;

Doi, H ;

Ko, MSH ;

Pontius, J ;

Schriml, L ;

Wagner, L ;

Maglott, D ;

Brown, SDM ;

Lander, ES ;

Schuler, G ;

Denny, P .

NATURE GENETICS, 2001, 29 (02) :201-205

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