Quantitative retention-structure and retention-activity relationship studies of local anesthetics by micellar liquid chromatography

The retention of compounds in micellar liquid chromatography (MLC) is governed by hydrophobic and electrostatic forces, For ionic compounds, both interactions should be considered. The present report offers a novel retention model that includes the hydrophobicity of compounds and the molar fraction of the charged form of compounds and compares it with other previously reported models. High correlations between the logarithm of capacity factors and these structural parameters were obtained or local anesthetics with different degrees of ionization using a nonionic surfactant solution as mobile phase, Modeling the retention of compounds as a function of physicochemical parameters and experimental variables is established by means of multiple linear regression. In addition, a predictive model for estimating the hydrophobicity of local anesthetics is proposed. Finally, quantitative and qualitative retention-activity relationships in MLC are also investigated for these compounds. An excellent correlation between the capacity factors in MLC and the anesthetic potency of local anesthetics was obtained.