Partial purification and characterization of protease of Bacillus proteolyticus-CFR3001 isolated from fish processing waste and its antibacterial activities

被引：129

作者：

Bhaskar, N. ^{[1
]}

Sudeepa, E. S.

Rashmi, H. N.

Selvi, A. Tamil

机构：

[1] Cent Food Technol Res Inst, Dept Meat Fish & Poultry Technol, Mysore 570020, Karnataka, India

[2] Cent Food Technol Res Inst, Dept Human Resource Dev, Mysore 570020, Karnataka, India

来源：

BIORESOURCE TECHNOLOGY | 2007年 / 98卷 / 14期

关键词：

alkaline protease; Bacillus proteolyticus; partial purification; antibacterial activity;

D O I：

10.1016/j.biortech.2006.09.033

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Bacillusproteolyticus CFR3001 isolated from fish processing wastes (both fresh water and marine) produced an alkaline protease. The optimum conditions for cell growth and protease production were 37 degrees C, 96 h, agitation speed of 100 rpm and medium pH 9. The partially purified protease obtained from had specific activity of 22.05 at 37 degrees C was active between 40 degrees C and 50 degrees C and lost > 20% of its activity around 60 degrees C. Its molecular weight was similar to 29 kDa and it inhibited the growth of several pathogenic organisms such as Escherichia coli, Listeria monocytogenes, Bacillus cereus and Yersinia enterocolytica. The scanning electron microscopy (SEM) studies revealed that the protease produced by B. proteolyticus CFR3001 lysed the cells of these pathogenic bacteria. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2758 / 2764

页数：7

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