Renal and systemic pH imaging by contrast-enhanced MRI

被引:164
作者
Raghunand, N
Howison, C
Sherry, AD
Zhang, SR
Gillies, RJ
机构
[1] Univ Arizona, Hlth Sci Ctr, Canc Ctr Div, Tucson, AZ USA
[2] Univ Texas, Dept Chem, Richardson, TX 75083 USA
[3] Univ Texas, SW Med Ctr, Rogers Magnet Resonance Ctr, Dallas, TX 75230 USA
关键词
gadolinium; kidney; relaxivity; acetazolamide; acid-base;
D O I
10.1002/mrm.10347
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Perturbations of renal and systemic pH accompany diseases of the kidney, such as renal tubular acidosis, and the ability to image tissue pH would be helpful to assess the extent and severity of such conditions. A dual-contrast-agent strategy using two gadolinium agents, the pH-insensitive GdDOTP5- and the pH-sensitive GdDOTA-4AmP(5-), has been developed to generate pH maps by MRI. The renal pharmacokinetics of the structurally dissimilar pH-insensitive contrast agents Gd-DTPA(2-) and GdDOTP5- were found to be similar. On that basis and on the basis of similarity of structure and charge, the renal pharmacokinetics of GdDOTp(5-) and GdDOTA-4AmP(5-) were assumed to be identical. Dynamic T-1-weighted images of mice were acquired for 1 hr each following boluses of GdDOTP5- and GdDOTA-4AmP(5-). The time-varying apparent concentration of GdDOTP5- and the time-varying enhancement in longitudinal relaxation rate following GdDOTA-4Amp(5-) were calculated for each pixel and used to compute pH images of the kidneys and surrounding tissues. MRI pH maps of control mice show acidic regions corresponding to the renal papilla, calyx, and ureter Pretreatment of mice with the carbonic anhydrase inhibitor acetazolamide resulted in systemic metabolic acidosis and accompanying urine alkalinization that was readily detected by this dual-contrast-agent approach. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:249 / 257
页数:9
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