Analysis of aqueous 2,4,6-trinitrotoluene (TNT) using a fluorescent displacement immunoassay

被引：47

作者：

Goldman, ER ^{[1
]}

Anderson, GP

Lebedev, N

Lingerfelt, BM

Winter, PT

Patterson, CH

Mauro, JM

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

[2] George Mason Univ, Fairfax, VA 22030 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2003年 / 375卷 / 04期

关键词：

environmental monitoring; seawater; explosives; fluorescent displacement immunoassay; 2,4,6-trinitrotoluene (TNT);

D O I：

10.1007/s00216-002-1713-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a rapid, simple, and sensitive assay that is potentially amenable to high throughput screening for analysis of 2,4,6-trinitrotoluene (TNT) present in aqueous solutions. The assay is based on the change in fluorescence emission intensity of a fluorescently labeled TNT analogue pre-bound to an anti-TNT antibody that occurs upon its competitive displacement by TNT. The assay can be performed in both cuvette- and 96-well plate-based formats. TNT at a level of 0.5 mug L-1 (0.5 ppb) was detected in phosphate buffered saline; detection improved to 0.05 mug L-1 (0.05 ppb) for TNT dissolved in artificial sea-water.

引用

页码：471 / 475

页数：5

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