Analysis of the complete genome sequence of black queen-cell virus, a picorna-like virus of honey bees

被引：105

作者：

Leat, N ^{[1
]}

Ball, B ^{[1
]}

Govan, V ^{[1
]}

Davison, S ^{[1
]}

机构：

[1] Univ Western Cape, Dept Microbiol, ZA-7535 Bellville, Cape Town, South Africa

来源：

JOURNAL OF GENERAL VIROLOGY | 2000年 / 81卷

关键词：

D O I：

10.1099/0022-1317-81-8-2111

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A virus with picorna-like biophysical properties was isolated from South African honey bees. On the basis of serology, it was identified as an isolate of black queen-cell virus (BQCV). Nucleotide sequence analysis revealed an 8550 nt polyadenylated genome containing two large ORFs. The 5'-proximal ORF (ORF 1) represented 4968 nt while the 3'-proximal ORF (ORF 2) represented 2562 nt. The ORFs were separated by a 208 nt intergenic region and were Ranked by a 657 nt 5'-untranslated region and a 155 nt 3'-untranslated region. Deduced amino acid sequences for ORF 1 and ORF 2 were most similar to the non-structural and structural proteins, respectively, of Drosophila C virus (DCV), Rhopalosiphum padi virus (RhPV), Himetobi P virus (HiPV) and Plautia stali intestine virus (PSIV), It is proposed that BQCV belongs to the group of picorna-like, insect-infecting RNA viruses constituted by DCV, RhPV, HiPV and PSIV.

引用

页码：2111 / 2119

页数：9

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