Fundamental properties of isoelectric buffers for capillary zone electrophoresis

被引:34
作者
Stoyanov, AV
Righetti, PG
机构
[1] Russian Acad Sci, Inst Gene Biol, Moscow 117334, Russia
[2] Univ Verona, Dept Agr & Ind Biotechnol, I-37134 Verona, Italy
关键词
buffer composition; ampholytes; isoelectric buffers; buffering power conductivity ratio;
D O I
10.1016/S0021-9673(97)00737-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Different isoelectric buffers are analysed theoretically, taking into account a fundamental parameter, i.e., the ratio between intrinsic buffering power and conductivity (R = beta/lambda). For a model ampholyte, the above parameter is analysed both as a function of the pI and the pK(b)-pK(a) values. For natural pH gradients, the variation of R, connected with approaching the isoelectric point, is evaluated. A case of oligo-protic ampholytes is also considered. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:169 / 176
页数:8
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