A simple method for detecting up to five immunofluorescent parameters together with DNA staining for cell cycle or viability on a benchtop flow cytometer

被引：22

作者：

Gordon, KM

Duckett, L

Daul, B

Petrie, HT

机构：

[1] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA

[2] BD Biosci, San Jose, CA 95131 USA

[3] Cornell Univ, Weill Grad Sch Med Sci, New York, NY 10021 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2003年 / 275卷 / 1-2期

关键词：

flow cytometry; cell cycle analysis; DNA staining; immunofluorescence;

D O I：

10.1016/S0022-1759(03)00009-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this manuscript, we describe modifications to a commercial three-laser benchtop flow cytometer, as well as relevant biological methods, that allow analysis of up to five immunofluorescent parameters together with an ultraviolet (UV)-excitable DNA stain. This method allows expanded capacity for multiparameter immunophenotyping of complex mixed cell populations, together with accurate measurements of DNA content (cell cycle) or cell viability, on a stable, end-user operated platform. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：113 / 121

页数：9

共 13 条

[1] RAPID, SIMULTANEOUS MEASUREMENT OF DNA, PROTEIN, AND CELL VOLUME IN SINGLE CELLS FROM LARGE MAMMALIAN-CELL POPULATIONS [J].