Membrane protein structural biology: the high throughput challenge

被引：130

作者：

Loll, PJ ^{[1
]}

机构：

[1] Drexel Univ, Coll Med, Dept Biochem, Philadelphia, PA 19102 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2003年 / 142卷 / 01期

基金：

美国国家卫生研究院; 美国国家航空航天局;

关键词：

crystallization; detergent; membrane protein; overexpression;

D O I：

10.1016/S1047-8477(03)00045-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane proteins represent roughly one-third of the proteins encoded in the genome, yet fewer than 1% of the proteins of known structure. High-throughput crystallography offers the hope of correcting this imbalance. In order for large-scale membrane protein structural biology to realize its full promise, however, significant technical challenges must be overcome, the two most substantial being facile protein overexpression and reliable methods for crystal growth. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：144 / 153

页数：10

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