Isothermal Detection of DNA by Beacon-Assisted Detection Amplification

被引：141

作者：

Connolly, Ashley R. ^{[1
]}

Trau, Matt ^{[1
]}

机构：

[1] Univ Queensland, Ctr Biomarker Res & Dev, AIBN, Brisbane, Qld 4072, Australia

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 15期

关键词：

biosensors; DNA detection; DNA nanomachines; molecular devices; nanobiotechnology; STRAND DISPLACEMENT AMPLIFICATION; NUCLEIC-ACIDS; POLYMERASE; REPLICATION; INVITRO;

D O I：

10.1002/anie.200906992

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Not bad at all: A simple biological circuit composed of two integrated molecular switches was developed for the detection and amplification of a specific nucleic acid in a biological extract. The nucleic acid is recognized by a molecular beacon, then a DNA polymerase and nicking endonuclease are used to rapidly amplify the signal for sensitive detection of the nucleic acid. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2720 / 2723

页数：4

共 16 条

[1] High accuracy genotyping directly from genomic DNA using a rolling circle amplification based assay [J].

Alsmadi, OA ;

Bornarth, CJ ;

Song, WM ;

Wisniewski, M ;

Du, J ;

Brockman, JP ;

Faruqi, AF ;

Hosono, S ;

Sun, ZY ;

Du, YF ;

Wu, XH ;

Egholm, M ;

Patricio, A ;

Lasken, RS ;

Driscoll, MD .

BMC GENOMICS, 2003, 4 (1)

[2] NUCLEIC-ACID SEQUENCE-BASED AMPLIFICATION [J].

COMPTON, J .

NATURE, 1991, 350 (6313) :91-92

[3] ROLLING REPLICATION OF SHORT DNA CIRCLES [J].

FIRE, A ;

XU, SQ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) :4641-4645

[4] ISOTHERMAL, INVITRO AMPLIFICATION OF NUCLEIC-ACIDS BY A MULTIENZYME REACTION MODELED AFTER RETROVIRAL REPLICATION [J].

GUATELLI, JC ;

WHITFIELD, KM ;

KWOH, DY ;

BARRINGER, KJ ;

RICHMAN, DD ;

GINGERAS, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (05) :1874-1878

[5] Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction [J].

Guo, Qiuping ;

Yang, Xiaohai ;

Wang, Kemin ;

Tan, Weihong ;

Li, Wei ;

Tang, Hongxing ;

Li, Huimin .

NUCLEIC ACIDS RESEARCH, 2009, 37 (03)

[6] Enzymatic signal amplification of molecular beacons for sensitive DNA detection [J].

Li, Jianwei Jeffery ;

Chu, Yizhuo ;

Lee, Benjamin Yi-Hung ;

Xie, Xiaoliang Sunney .

NUCLEIC ACIDS RESEARCH, 2008, 36 (06)

[7]

LU YY, 1991, BIOTECHNIQUES, V11, P464

[8]

MEAD DA, 1991, BIOTECHNIQUES, V11, P76

[9] SPECIFIC ENZYMATIC AMPLIFICATION OF DNA INVITRO - THE POLYMERASE CHAIN-REACTION [J].

MULLIS, K ;

FALOONA, F ;

SCHARF, S ;

SAIKI, R ;

HORN, G ;

ERLICH, H .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1986, 51 :263-273

[10] Isothermal reactions for the amplification of oligonucleotides [J].

Van Ness, J ;

Van Ness, LK ;

Galas, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (08) :4504-4509

← 1 2 →